On Mon, Dec 8, 2014 at 7:24 PM, Keller, Jacob <kell...@janelia.hhmi.org> wrote:
> FYI, sometimes native nucleotides can make it through protein > purifications if binding is tight. > This is especially true for G-proteins, since tight binding to GDP is an essential part of their function. I don't know what to expect from E. coli proteins, but human Ras co-purifies with GDP at close to 1:1. At low resolution, it might be easiest to superimpose the closest nucleotide-bound homolog and see how well the density aligns with the nucleotide. -Nat
