This is where it’s customary to include a small image or two (or better, a link thereto) which shows the density, and the masters here can tell you there best guesses—seems to be a bit of a parlour game. Also include info on what is in the crystallization condition and protein buffer if you dare. FYI, sometimes native nucleotides can make it through protein purifications if binding is tight.
JPK From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Yamei Yu Sent: Monday, December 08, 2014 10:15 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] unknown densities Hi all, We crystallised a small GTPase expressed in E. Coli and found some densities in GDP/GTP binding site. We didn’t add any GDP/GTP or GDP/GPD homologues during protein expression, purification and crystallisation. The resolution is not high, we couldn’t tell what it is. Is there any method to detect what it is? Thanks! Best wishes! yamei ************************************************ Yamei Yu State Key Laboratory of Biotherapy／Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University,Chengdu,610041, P.R.China Tel: 15882013485 Email: ymyux...@gmail.com<mailto:ymyux...@gmail.com> ymyux...@163.com<mailto:ymyux...@163.com> yamei...@scu.edu.cn<mailto:yamei...@scu.edu.cn>
