Re: [ccp4bb] ACA 2014 meeting--Session on engaging students in protein crystallography

2014-01-14 Thread Jrh
Dear Roger, I have regularly supervised MChem final year students in their inorganic chemistry, and in earlier years physics students, two semester , two days, per week protein crystallography projects. A very good and consistent theme involves metal atoms binding to proteins. With quite a few s

[ccp4bb] Circular Dichoism 280nm range signal

2014-01-14 Thread JinSoo.Bae
Dear all, Sorry for the off topic. We are comparing the high order structure between Fc engineered mAbs using CD methods. There are some different (noise ?) signal in the 280nm range each antibodies. Other region is almost close match with respect of peak shape and theta value. Is there any s

Re: [ccp4bb] ACA 2014 meeting--Session on engaging students in protein crystallography

2014-01-14 Thread Jim Pflugrath
Hi Roger, We have a simple lysozyme project where we collect diffraction images in less than 30 seconds total exposure time and solve the structure by SAD phasing. I've thought that this might make an ideal lab practical: grow crystals one week, everyone collects the data on their own crystal

Re: [ccp4bb] autosharp error message

2014-01-14 Thread Clemens Vonrhein
Dear Almudena, On Tue, Jan 14, 2014 at 07:34:21PM +0100, Almudena Ponce Salvatierra wrote: > Dear all, > > I find this error message while running autosharp: > > No column "FP" of type "F" found in file >/usr/local/sharp/users/sharp/None.sharp/datafiles/xxx.mtz! > > Does anybody kno

Re: [ccp4bb] refining nucleic acids with Coot

2014-01-14 Thread Almudena Ponce Salvatierra
Thank you all very much for your very helpful advices! I managed to solve my problem! :-) Best, Almudena. 2014/1/14 Ashley Pike > Hi Almudena – I came across this issue recently as well with an old pdb > file. As Paul says you need to convert from v2.3 to v3.2 pdb formats. In > addition to t

[ccp4bb] autosharp error message

2014-01-14 Thread Almudena Ponce Salvatierra
Dear all, I find this error message while running autosharp: No column "FP" of type "F" found in file /usr/local/sharp/users/sharp/None.sharp/datafiles/xxx.mtz! Does anybody know how to solve it? I have tried several things but they did not work. I am quite new with this software so a

Re: [ccp4bb] refining nucleic acids with Coot

2014-01-14 Thread Paul Emsley
On 14/01/14 15:51, Almudena Ponce Salvatierra wrote: Dear all, I am trying to refine an oligonucleotide chain in Coot and I get the following message when I try: "Fail to match (to the dictionary) the following model atom names: G O5* C5* O4* etc That would cause exploding atoms so the re

[ccp4bb] refining nucleic acids with Coot

2014-01-14 Thread Almudena Ponce Salvatierra
Dear all, I am trying to refine an oligonucleotide chain in Coot and I get the following message when I try: "Fail to match (to the dictionary) the following model atom names: G O5* C5* O4* etc That would cause exploding atoms so the refinement did not start" Does anyone know how to solve th

[ccp4bb] ACA 2014 meeting--Session on engaging students in protein crystallography

2014-01-14 Thread Roger Rowlett
I am co-organizing (with Kraig Wheeler) a session at the 2014 American Crystallographic Association Meeting in Albuquerque, NM concerned with engaging undergraduate students in protein crystallography. I would like to encourage anyone who has involved undergraduate students in protein crystallo

[ccp4bb] 2nd CCP4/CeBEM South American School in Macromolecular Crystallography

2014-01-14 Thread Ronan Keegan
Dear Colleagues, We are pleased to announce the second CeBEM/CCP4 South American School in Macromolecular Crystallography. This year's school will take place at Sao Carlos University in Brazil. Full details including the program and application information can be found at the school website:

[ccp4bb] PhD positions in NMR spectroscopy/structural biology of photosynthetic membrane proteins at Leiden University, The Netherlands

2014-01-14 Thread Anjali Pandit
Currently, PhD positions are available at Leiden University, Leiden Institute of Chemistry, within the project "in shape for photoregulation: molecular plasticity in photosynthesis" financed by a VIDI grant of the Netherlands Organization of Scientific Research. *Job description* The solid-st

Re: [ccp4bb] Assays for protein-ligand interaction?

2014-01-14 Thread Jia-Ying Guan
You can try MST(MicroScale Thermophoresis), if there is an instrument available. It is like a mini ITC, but much more versatile. The sample consumption is very low, claimed by the company that sells the instrument. http://www.nanotemper-technologies.com/technology/microscale-thermophoresis/article/