Pascal,
Not personally observed this myself, but just wondering, as a control, have you
tried setting trays with just the buffer+detergent (no PDC)? And if so, do you
see the same behavior or not? Also, do you see phase separation in all drops
or just a majority? Do the drops that exhibit th
Dear CCP4,
Sorry for the off-topic post but we wanted to make the community aware that we
are running a significant sale on our fragment library 1 to help offset the
cuts to NIH due to the sequester. Library 1 is our first pass library, is a
good stand-alone library and comes as a screening ki
Dear All,
I have a question tailored for the membrane protein and detergent folks. We
are purifying a membrane protein that associates into an homoligomeric pore
and we have been successfully preparing it in two detergents: FC-12 or a
mild lipid. The two Protein Detergent Complexes look very homog
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Dear Pietro,
would this not look like a frame from your home source with no crystal
mounted - unless you do not have access to a source where you can
quickly collect an exposure in the absence of a crystal, but from your
email address I assume this is
Dear all,
together with two fellow crystallographers,
I am writing a pamphlet to introduce schoolchildren
to X-ray crystallography.
For the introductory chapter,
we would need a picture of X-rays scattered by air.
Or by any gas for that matter.
I have tried Google images without much luck.
Can
wwPDB Workshop on mmCIF/PDBx for Programmers
What, why and how?
--
The world of the PDB will be changing rapidly and profoundly over the next few
years. A major change will involve the transition from PDB to mmCIF/PDBx as
the principa
Folks
The latest edition of the Computational Crystallography Newsletter is
available at http://phenix-online.org/newsletter
The table of contents is listed below.
Articles
*cctbx* tools for transparent parallel job execution in Python. III. Remote
access
*phenix.ensemble_refinement*: a test s
Hi ,
Seems to me that crystal packing is not good thats why mosaic
diffraction, this might be due to DNA so you can increase diffraction
quality by using 1 or 2 nucleotide sticky end DNA for crystallization
which can help in crystal packing .
Vandna Kukshal
Postdoctral Research Associate
De
Thank you all for your helps,
It seems that either COOT with modified PDB file or PDBSET is able to do
most of the tricks for me. And thank Edward for sharing you useful files! I
have to do a little bit more homework on xtaldraw though.
Chen
On Thu, Aug 1, 2013 at 9:17 AM, Edward A. Berry wrote
Hi Appu,
nothing really to worry about if you process your data with XDS or d*trek using
3D profile fitting.
You still should be able to get something useful out of this data.
If you look at 90.png (I'm attaching a zoomed area of your image), you see your
crystal is either split or you had two c
Dear Appu,
one direction of your cristal has very anistropic diffraction. I think
your cristal has grown like multi-layer. The problem is probably due to
the DNA. If the different "layer" of your cristal are not very well
aligned, you have this type of problem.
In my opinion, you can try to
That looks like a garden variety mosaic/kinda-crappy crystal. Without being
there, it's impossible to tell if it was morphology (e.g. cracks and divots,
etc.), crystal handling, or just bad luck. The bottom line is you need better
data. The good news is that those crystals diffract to a reasonab
It's not ideal but we have been using a program called XtalDraw on windows for this, to display the different lattices
and to build up the content of the unit cell from a small molecule of 2 or 3 atoms by applying the different space group
symmetries. The students can edit the coordinates file (n
Tim has the more modern answer, but PDBSET
http://www.ccp4.ac.uk/html/pdbset.html is quite simple and scriptable.
If I recall correctly, the SYMGEN keyword may be of use.
>From the example in the documentation:
Expand dimer to tetramer, rename chains, transform
#!/bin/cs
In our experience, "dry shipper" dewars will "fail" for two reasons:
1) the vacuum gets compromized
2) the "sponge" that soaks up the liquid nitrogen gets contaminated with
water
Hot and cold cycling can cause the vacuum pump-out valve (that
sticky-out thingy that always seems to be in the way
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Dear Chen Zhao,
if I understand correctly, you can use coot: It displays the unit cell
based on the CRYST1 card present in some PDB file, and can read in and
display the PDB file and also show the symmetry equivalents. It also
writes out the coordinat
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