That looks like a garden variety mosaic/kinda-crappy crystal. Without being there, it's impossible to tell if it was morphology (e.g. cracks and divots, etc.), crystal handling, or just bad luck. The bottom line is you need better data. The good news is that those crystals diffract to a reasonably good resolution on your home source. The bad news is that the data you have in hand are probably not that useful. It might be a case of just trying 50 crystals before you get a good one or you might have to modify your cryo, crystal growth, etc.
Good luck. _____________________________________________ Eric A. Toth, Ph.D. Assistant Professor Department of Biochemistry and Molecular Biology Marlene and Stewart Greenebaum Cancer Center University of Maryland School of Medicine 108 North Greene St. Baltimore, MD 21201 Email: [email protected]<mailto:[email protected]> Phone: x-410-706-5345 Fax: x-410-706-8297 http://medschool.umaryland.edu/FACULTYRESEARCHPROFILE/viewprofile.aspx?id=8032 http://crystal.umaryland.edu<http://crystal.umaryland.edu/> [Description: Description: Description: UMSOM New logo] A Third Century Where Discovery Transforms Medicine From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Appu kumar Sent: Thursday, August 01, 2013 9:45 AM To: [email protected] Subject: [ccp4bb] Spots not getting indexed properly in protein-DNA complex Dear all ccp4 user, We have collected a data set of 370 frames with 0.5 OSC for a protein-DNA complex on RAXIS IV detector. The spots look mostly clustered from 50A to 6A region but the whole data is completely spreaded to 2.8A. There are many spots which are very near to each other as if they were merged but closely placed. When we are trying to index the data, its not picking all the spots correctly and giving a unit cell dimension variable from 150 to 500A in one of the axis. Rest two axis axes of unit cell are almost similar ~ 55A, 111A. We tried processing with HKL2000 but not able to index it., I am attaching four 4 images for every 90 degree frames collected. Please look at these images and give your valuable input regarding indexing problem. your suggestions and support will be highly appreciated. Please guide me and help me sorting out the problem. Thank you
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