Hi Appu, nothing really to worry about if you process your data with XDS or d*trek using 3D profile fitting. You still should be able to get something useful out of this data. If you look at 90.png (I'm attaching a zoomed area of your image), you see your crystal is either split or you had two crystals in the loop. Look at the second row of reflections when starting from the beam stop position. Since the first and third row does not follow the same trend, I would think you have a second crystal in a slightly different orientation grown with the main crystal. [cid:4DB7FA0D-B1B8-44C0-A7DD-B087B0E83914@sph.ad.jhsph.edu] You should not try to index on all reflections, try getting one single lattice and ignore the other one. You will run into overlapping issues from the two lattices but you might still get good enough data to try MR when you process with XDS. With XDS you have the advantage of collecting all strong reflections over the whole dataset and then index on those.
You can download it from here: http://xds.mpimf-heidelberg.mpg.de Jürgen ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu On Aug 1, 2013, at 9:44 AM, Appu kumar wrote: Dear all ccp4 user, We have collected a data set of 370 frames with 0.5 OSC for a protein-DNA complex on RAXIS IV detector. The spots look mostly clustered from 50A to 6A region but the whole data is completely spreaded to 2.8A. There are many spots which are very near to each other as if they were merged but closely placed. When we are trying to index the data, its not picking all the spots correctly and giving a unit cell dimension variable from 150 to 500A in one of the axis. Rest two axis axes of unit cell are almost similar ~ 55A, 111A. We tried processing with HKL2000 but not able to index it., I am attaching four 4 images for every 90 degree frames collected. Please look at these images and give your valuable input regarding indexing problem. your suggestions and support will be highly appreciated. Please guide me and help me sorting out the problem. Thank you <90.png><180.png><250.png><371.png><0.png>
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