Dear Umri,
I think the main problem is co-crystallization.
What I would do is crystallize protein and antibody separately and then soak
protein crystals into reservoir solution containing antibody or vice versa.
And do try to get crystals from different conditions which may alter the space
grou
Is this Laue diffraction in the sense that the neutrons are a spectrum of
energies, or does Laue mean something else here?
Jacob
On Thu, May 23, 2013 at 3:53 PM, Meilleur, Flora wrote:
> IMAGINE, Neutron Crystallography Diffractometer
>
> High Flux Isotope Reactor (HFIR), Oak Ridge Nationa
This should be your first highlight to NSF - and he should see it today/tomorrow
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Meilleur,
Flora
Sent: Thursday, May 23, 2013 3:54 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Call for Proposals: IMAGINE, a New Neutron Crysta
ACA BioSAS Training Workshop WK.01
Biological Small Angle Solution Scattering
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2013 ACA meeting in Hawaii
There is still Room. Reserve your space now! The early registration discount
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Small angle solution scattering (SAS) has recently experienced a dramatic
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You are invited to apply for a postdoctoral position in the structural biology
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Dear Klaus
If your compound can be dried, you can add it to the drop as a powder
from the tip of a acupuncture needle. At least this works for many of
the insoluble heavy metal derivatives. We have also successfully
resuspended the powder in the optimal buffer of choice and added it as
insolub
I keep sending mails by accident today - apologies for the spam. The
last sentence of my should read:
This could of course be due to too high a concentration of mother
liquor but quite often it occurs at relative humidity values where the
concentration of the mother liquor components will not
Hi Ed,
good question. I have found that you have a good 30 seconds to remove
the surrounding liquid - so while you have to do it fast you have enough
time that it doesn't need a robot and even a malcoordinate such as
myself can do it. I'm afraid that I have no estimate for how often
diffractio
Dear Klaus,
We've had a lot of luck with ethylene glycol and isopropanol, using them
successfully in cases where DMSO affects diffraction.
Best wishes
Richard
=
Dr Richard Bayliss, Reader in Structural Biology
Department of Biochemistry
Henry Wellcome Building
Univer
Hi Klaus,
- small molecular weight PEG's e.g. 200 instead of DMSO, has the advantage of
also helping to cryo protect
- Methanol (only for dispensing the compound into wells) then allow to
evaporate and simply add your cryo-protected crystals, the hope is that
sufficient of your ligand goes into
Dear CCP4BB followers,
We are currently trying to obtain ligand-bound complexes for one of our
proteins by soaking and/or co-crystallisation. We have had prior success for
this protein, but using a different class of ligands. The new ligand (in DMSO)
remains in solution (more or less) when mi
Matt,
with this technique, how do you prevent crystal from drying up (other
than "doing it fast")? I know Thorne's group does this trick under oil.
If you take no extra precautions, do you have an estimate of how often
diffraction is destroyed by this?
On the other hand, it's quite possible th
L-proline works well with ammonium sulfate:
http://www.ncbi.nlm.nih.gov/pubmed/22868767
Sent from Jack's iPad
On May 23, 2013, at 4:42 AM, "Faisal Tarique" wrote:
>
> Dear all
>
> Can anybody tell me the appropriate cryo condition for the crystals obtained
> in 2M Ammonium sulphate and tri
Just to follow up, the paper that was attached is based on the far more
original work by Garman and Mitchell
Elspeth Garman and Edward Mitchell. (1996) Glycerol concentrations required for
cryoprotection of 50 typical protein crystallisation solutions. J.Appl. Cryst.
29, 584-587.
And there is
The version of ADXV we have has some nice resolution ring features.
There is a mode with the typical 5 rings of evenly spaced resolution.
There is "Anchor1" which draws a circle about the beam centre and
through a selected point. There is "Pick3" which constructs a circle
from 3 selected points
Hi Faisal,
if your solvent channels are smaller than 40A in the largest dimension
(most are) you can use a mesh loop to pick up the crystal and then wick
away all of the mother liquor. You can then flash cool your crystal
without having to transfer the crystal to another solution. Good luck,
M
Hi Faisal,
On 2013-05-23 11:42, Faisal Tarique wrote:
Dear all
Can anybody tell me the appropriate cryo condition for the crystals
obtained in 2M Ammonium sulphate and tris pH8.5..I tried to add 10%
glycerol to it but still the ice ring is forming..
Thanx in advance--
Regards
Faisal
School o
Hi Faisal,
3 - 3.5M Ammonium sulphate with your buffer should work too.
Take a small loop.
Jan
--
Jan Abendroth
Emerald BioStructures
Seattle / Bainbridge Island WA, USA
home: Jan.Abendroth_at_gmail.com
work: JAbendroth_at_embios.com
http://www.emeraldbiostructures.com
On May 23, 2013, at 2:42 AM
I would try 2.5M or 3M sodium malonate pH 7
Janet Newman
Principal Scientist / Director, Collaborative Crystallisation Centre
CSIRO Material Science and Engineering
343 Royal Parade
Parkville. VIC. 3052
Australia
Tel +613 9662 7326
Email janet.new...@csiro.au
From
30% glycerol or 25% glucose should be sufficient for 1-2 M ammonium sulfate.
Roger Rowlett
On May 23, 2013 5:42 AM, "Faisal Tarique" wrote:
>
> Dear all
>
> Can anybody tell me the appropriate cryo condition for the crystals
> obtained in 2M Ammonium sulphate and tris pH8.5..I tried to add 10%
>
Dear bulletin board,
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Hi,
Paraton oil worked nicely for me for these conditions.
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
80% saturated Li2SO4
On Thu, 23 May 2013 11:42:09 +0200, Faisal Tarique
wrote:
Dear all
Can anybody tell me the appropriate cryo condition for the crystals
obtained in 2M Ammonium sulphate and tris pH8.5..I tried to add 10%
glycerol to it but still the ice ring is forming..
Thanx in adva
Dear all
Can anybody tell me the appropriate cryo condition for the crystals
obtained in 2M Ammonium sulphate and tris pH8.5..I tried to add 10%
glycerol to it but still the ice ring is forming..
Thanx in advance
--
Regards
Faisal
School of Life Sciences
JNU
Phenix has a diffraction viewer, too.
You may want to try that.
Ciao,
Sebastiano
--
Sebastiano Pasqualato
Crystallography Unit
Department of Experimental Oncology
European Institute of Oncology
via Adamello, 16
Milan, Italy
On 23/mag/2013, at 10:16, Rafal Dolot wrote:
> Dear CCP4 users,
>
>
Photoshop?
A.
Sent from my iPad
On 23 May 2013, at 10:16, Rafal Dolot wrote:
> Dear CCP4 users,
>
> I'm looking for the diffraction image viewer, which will be able to
> display of resolution circles and export it to new image. I tried use
> idiffdisp, but after choose of the "Show/clear res
iMosflm can certainly do this for you. You might have to screen grab to capture
the resolution rings though.
Tony.
On 23 May 2013, at 09:27, "Rafal Dolot" wrote:
> Dear CCP4 users,
>
> I'm looking for the diffraction image viewer, which will be able to
> display of resolution circles and ex
Hi Rafal,
have you try ADXV ?
I never try to use idiffdisplay, but i know that ADXV can display
resolution circles.
If your software is able to open the image, but can't show the circles.
I think it's not really a problem with the image format, but with the
information in the Header of your
Dear CCP4 users,
I'm looking for the diffraction image viewer, which will be able to
display of resolution circles and export it to new image. I tried use
idiffdisp, but after choose of the "Show/clear resolution circles", there
is no action. Images were collected using Rayonics MX-225 detector -
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