Yes, it is very common. I solved two of them at 2-3 A resolution and
saw my colleague had one with 1.4 A resolution (can't remember the
name of the protein).
Please see references
J Med Chem. 2010 Jul 22;53(14):5229-39. doi: 10.1021/jm100377f.
Complexes of bacterial nicotinate mononucleotide aden
On Monday, January 07, 2013 12:10:17 pm Edward A. Berry wrote:
> The idea is (whether it's valid or not) to apply the information from
> both sites simultaneously. If the density is pretty ambiguous and one side
> tends to drift off into an alternate conformation and the other drifts off
> into ano
The idea is (whether it's valid or not) to apply the information from
both sites simultaneously. If the density is pretty ambiguous and one side
tends to drift off into an alternate conformation and the other drifts off
into another conformation, but you have every reason to believe that
the confo
On Mon, 07 Jan 2013 18:30:32 +0100, Joy wrote:
Dear Bryan,
Thank you very much for the comments, I have tried glycerol and it did
affect the resolution, at the same time, through reading former posts, I
find that sucrose might not be a good choice for crystals grown in salt,
I will defi
Dear Enrico,
Thank you very much for the comments, will definitely try it later.
Best,
Joy
-Original Message-
From: Enrico Stura [mailto:est...@cea.fr]
Sent: 2013年1月7日 10:16
To: CCP4BB@jiscmail.ac.uk; Joy
Subject: Re: [ccp4bb] Ammonium phosphate as cryoprotectant
Joy,
Try 80% satura
Dear Bryan,
Thank you very much for the comments, I have tried glycerol and it did affect
the resolution, at the same time, through reading former posts, I find that
sucrose might not be a good choice for crystals grown in salt, I will
definitely try adding glycerol stepwise, but at the same
Just a naive question: why apply NCS to ligands at all? Their contribution to
the number of parameters and hence to the param/obs ratio, the main argument
for applying NCS, is negligible, isn't it?
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84
On Mon, Jan 7, 2013 at 1:28 AM, Xiaopeng Hu wrote:
> We recently resolved an enzyme/inhibitor complex structure. The enzyme
> contains two NCS related active site and we did find extra density in both of
> them.However we observed that the two inhbitor moleculors are not NCS
> related, but part
But I think the original poster meant partially overlapped after applying
the ncs- operator- i.e. they are not ncs related but occupy partly the same
position (in the two non-overlapping copies of the binding site).
Then I guess it depends how clear the density is- If the density is not very
clea
Joy,
Try 80% saturated Lithium sulfate (2.5M) instead.
Just transfer the crystals and if it does not work
let me have more precise crystallization conditions and I will post you
other options.
Enrico.
On Thu, 03 Jan 2013 18:33:05 +0100, Joy wrote:
Dear All,
May I have your expert opini
Dear All,
Something like what Felix wrote is seen in the crystal structure of recombinant
human acetylcholinesterase (rhAChE) (PDB-ID: 3lii), with two molecules are seen
in the asymmetric unit.
* In one molecule, the active-site gorge (where inhibitors normally lie) is
occupied with part of a pe
I apologise for typing blinbly:
" if one is in, the second can't be"
FF
Dr Felix Frolow
Professor of Structural Biology and Biotechnology, Department of Molecular
Microbiology and Biotechnology
Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor
e-mail: mbfro...@post.tau.ac.
Why not? They can be mutually excluding! If one is in, the second can be. This
phenomenon brakes a local symmetry.
FF
Dr Felix Frolow
Professor of Structural Biology and Biotechnology, Department of Molecular
Microbiology and Biotechnology
Tel Aviv University 69978, Israel
Acta Crystallograp
Dear All,
We recently resolved an enzyme/inhibitor complex structure. The enzyme contains
two NCS related active site and we did find extra density in both of
them.However we observed that the two inhbitor moleculors are not NCS related,
but partly overlaped if make a NCS moleculor. Has anyone
Thanks all for the quick replies! Thanks too for the gazillion
contributions to the long long thread - helping me win my bet that
people simply wouldn't be able to resist... :)
On 07/01/2013 06:51, Felix Frolow wrote:
###
#
Postdoctoral research position in Structural Biology of Asymmetric Cell Division
A postdoctoral research position is available in the group of Marina Mapelli at
the European Institute of Oncology in Milano
(http://www.ifom-ieo-campus.it/research/mapelli.php).
The main interest of the group focu
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