Dear Enrico, Thank you very much for the comments, will definitely try it later.
Best, Joy -----Original Message----- From: Enrico Stura [mailto:est...@cea.fr] Sent: 2013年1月7日 10:16 To: CCP4BB@jiscmail.ac.uk; Joy Subject: Re: [ccp4bb] Ammonium phosphate as cryoprotectant Joy, Try 80% saturated Lithium sulfate (2.5M) instead. Just transfer the crystals and if it does not work let me have more precise crystallization conditions and I will post you other options. Enrico. On Thu, 03 Jan 2013 18:33:05 +0100, Joy <joybeiy...@gmail.com> wrote: > Dear All, > > > May I have your expert opinion on the following question: what is the > minimum concentration of ammonium dibasic phosphate if I would like to > use it as cryoprotectant? > > > Happy New Year! > > > Joy > -- Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-sacl ay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-prote ins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallo genesis-e.-stura http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
