Dear Bryan,
Thank you very much for the comments, I have tried glycerol and it did affect the resolution, at the same time, through reading former posts, I find that sucrose might not be a good choice for crystals grown in salt, I will definitely try adding glycerol stepwise, but at the same time, I am wondering if high conc. ammonium phosphate could be used as cryoprotectant, if so, I would be able to achieve dehydration and cryo at the same time. Best, Joy From: Prince, D Bryan [mailto:dbryan.pri...@astrazeneca.com] Sent: 2013年1月3日 17:17 To: Joy Subject: RE: [ccp4bb] Ammonium phosphate as cryoprotectant Dear Joy, I do not believe that ammonium dibasic phosphate will make a good cryosalt. I would recommend a lithium cryosalt (formate, acetate or sulfate) or glycerol if you really need to stay with the phosphate. Hope this helps! Bryan _____ Confidentiality Notice: This message is private and may contain confidential and proprietary information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorized use or disclosure of the contents of this message is not permitted and may be unlawful. From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Joy Sent: Thursday, January 03, 2013 12:33 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Ammonium phosphate as cryoprotectant Dear All, May I have your expert opinion on the following question: what is the minimum concentration of ammonium dibasic phosphate if I would like to use it as cryoprotectant? Happy New Year! Joy
