Hi everybody,
I was doing a regular fink update (OS X 10.6.4, 32-bit fink) when I
encountered a silly outcome, which I thought others might have observed.
During the imosflm update, I get an error saying that imosflm depends on
mosflm, and it also conflicts with mosflm at the same time.
Thi
On Jul 4, 2010, at 20:35, chern wrote:
It's an interesting discussion.
1.Usually it's not possible to use mass-spec for non-covalent
complexes.
Native mass spec is well developed. I would hope to give you my
favorite reference,
but its for now pending, courtesy of some reviewers in Mol.
Hi,
'The Role of Diffusion in Enzyme Kinetics'
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1367934/pdf/biophysj00672-0040.pdf
Mark
~
Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry and
Drug Discovery,
Wel
Hi Jacob -
Sorry if I caused any misunderstanding, but I am not saying that kD is
not mattering in cells.
Of course it should matter.
I am making the distinction that the concentration dependent component
of it, k(on), is of less
importance, since in the viscous environment of a cell, the c
Yes, I know.
I am already working on some other modifications, or analogues. The
questions was only to know some experience from different labs. Thanks.
Petr
2010/7/5 Vellieux Frederic
> Petr Kolenko wrote:
>
>> Dear all,
>>
>> I am working on crystallization of a protein purified using intein-
Petr Kolenko wrote:
Dear all,
I am working on crystallization of a protein purified using
intein-chitin binding domain. There is 86% identity with protein with
already known high-resolution structure, but I am not able to get any
crystals. I heard that reversed phase chromatography is not goo
Dear all,
I am working on crystallization of a protein purified using intein-chitin
binding domain. There is 86% identity with protein with already known
high-resolution structure, but I am not able to get any crystals. I heard
that reversed phase chromatography is not good for crystallization, e.
