Dear all, I am working on crystallization of a protein purified using intein-chitin binding domain. There is 86% identity with protein with already known high-resolution structure, but I am not able to get any crystals. I heard that reversed phase chromatography is not good for crystallization, e.g. Has anyone of you observed something similar for intein-chitin chromatography? Is there any serious "structural" damage to the target protein during the purification?
-- Petr Kolenko kole...@imc.cas.cz http://kolda.webz.cz
