Petr Kolenko wrote:
Dear all,
I am working on crystallization of a protein purified using
intein-chitin binding domain. There is 86% identity with protein with
already known high-resolution structure, but I am not able to get any
crystals. I heard that reversed phase chromatography is not good for
crystallization, e.g. Has anyone of you observed something similar for
intein-chitin chromatography? Is there any serious "structural" damage
to the target protein during the purification?
--
Petr Kolenko
kole...@imc.cas.cz <mailto:kole...@imc.cas.cz>
http://kolda.webz.cz
The failure in crystallisation can be due to so many reasons...
including protein damage (ending up with a mixture of components is
probably one of the worse cases) Sometimes you need to modify your
protein in order to crystallise it, or even use an analog from another
organism.
Fred.
