Dear all:
I am trying to solve a structure from apparently a hexagonal crystal.
I indexed and scaled data in P6 in Scalepack (with merging) then used
Scalepack2mtz (with ensure unique reflections and add Rfree as well as
the truncate procedure), and then attempted to run molecular
replac
Hi All
I'm interested in screening small molecule libraries against my xtal
for potential binders. There are many papers that cover this (http://www.nature.com/nbt/journal/v18/n10/abs/nbt1000_1105.html
, for example).
If you have had experience in this field, please email me privately as
I
Hi Rui,
to get it work, I would make sure that I have the last version of X-code
tools, and update X-quartz with the last version 2.4.0, you can download it
here: http://xquartz.macosforge.org/trac/wiki
I would also update fink and the source list which are different for Intel
and PPC: check Bill
Dear All:
Thanks a lot for all the responses. My question was about measuring the
detergent concentration after concentrating a membrane protein sample.
Here is the summary.
1. The simplest way to control the detergent concentration is to use a
higher cut-off concentrator if you protein plus de
Good times :)
When you patent this design, be sure to give royalties to all the
contributors. Daddy needs a new Ferrari.
I assume you've seen Inteins already, right? Cleavage induced by DTT or BME.
Artem
> Hi,
>
> We would like to design a self-cleaving tag. It will be similar to the one
> Roge
Hi Daniel,
look at this
the Profinity eXact Fusion-tag system from BioRad* *
the protease i fused to your protein and self activated by halides (F or I I
think). Cleavage in on column. The principles is clever, now the cleavage
conditions may not suit to your protein, but it seems to work.
This i
Hi,
We would like to design a self-cleaving tag. It will be similar to the one
Roger Tsien has developed but I hope to find one that works in the opposite
way.
Lin, M.Z. et al. A drug-controllable tag for visualizing newly synthesized
proteins in cells and whole animals. Proc. Natl. Acad. Sci
Hi All,
I got a mac pro 10.5.8 and transferred all the programs from my old mac (
ppc ), now when I tried to run coot, it will start and crash with the
following error message:
Reading coordinate file: /sw/share/coot/standard-residues.pdb
PDB file /sw/share/coot/standard-residues.pdb has been re
Dear All,
for those who need an excuse to escape the winter chill - I have scheduled
a short-course on Biomolecular Crystallography/SBDD in San Diego/La Jolla.
Fee includes a copy of my book.
Details:
http://www.ruppweb.org/workshops/Molsoft_2009.htm
Best wishes, BR
---
On Tue, Oct 27, 2009 at 02:13:48PM +0100, Dirk Kostrewa wrote:
I agree with Ronnie Berntsson and Roger Rowlett. The only thing
that you
can't do on those machines is to work with Coot in stereo 3D.
Currently,
you would need a MacPro and a special Nvidia Quadro graphics card
(and
stereo glas
As Artem and Ezra have already mentioned the basic aspects of
isolating IBs, I should point out that most people wash the pellet of
the first low-speed centrifugation 2-3 more times. While Ezra
suggests using increasing amounts of urea, you may have to tailor the
composition of the wash bu
Yep, solved a structure at Cu K-alpha once with the sulfur signal,
resolution 1.7A and a redundancy of 50 or so did the trick. Actually,
one of the sulphurs we found was an ordered SO4 molecule, so if you have
that in your crystallisation condition it might be a bonus...
Flip
Watanabe Nobuhis
Artem has already responded - but I believe you will pull down the
cellular debris with the IB.
In my prior experience, post centrifugation, you can wash the IB/debris
with increasing amounts of urea in you solubilization - and you may find
that the other cellular debris stays in solution befo
Posted on behalf of Prof. Francesco Blasi, who might be directly
contacted if interested
(check http://www.ifom-ieo-campus.it/research/blasi.php for contacts)
Post doctoral position available from January 2010.
Laboratory: Prof. Francesco Blasi, IFOM (Foundation FIRC Institute of
Molecular
There's OpenMP parallelization of some of the most CPU-intensive parts of
molecular replacement in Phaser, in the versions released recently in
Phenix and hopefully in the next CCP4 release. However, OpenMP is not
turned on by default in the compilation of the binaries released with
Phenix, so
A company called ZoBio screens their 2,000 fragment library with NMR,
cool technology. Don't know about €
www.zobio.com
Flip
Sridhar Prasad wrote:
Zenobia is a good place to look for, they can even customize it your needs.
www.*zenobiatherapeutics*.com
Sridhar
> Date: Wed, 28 Oct 2
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