Good times :) When you patent this design, be sure to give royalties to all the contributors. Daddy needs a new Ferrari.
I assume you've seen Inteins already, right? Cleavage induced by DTT or BME. Artem > Hi, > > We would like to design a self-cleaving tag. It will be similar to the one > Roger Tsien has developed but I hope to find one that works in the > opposite way. > > Lin, M.Z. et al. A drug-controllable tag for visualizing newly synthesized > proteins in cells and whole animals. Proc. Natl. Acad. Sci. USA 105, > 7744â7749 (2008). > > Tsien used the NS3 protease which is inhibited by a cell-permeant drug. > The tag will be cleaved in the absence of this drug. I would like to find > a small and monomeric protease which is inactive until a drug or a > co-factor is applied. Any suggestions will be highly appreciated. > > Best, > Daniel > > > >
