Dear Len,
when you start ccp4i from the command line (a terminal), does it produce
any error messages?
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Tue, 9 Jun 2009, Leonard Thomas wrote:
I have ran into this problem before b
Hello,
We have a 2006 Rigaku Micromax-007H system available. Configuration is:
• MicroMax-007HF X-Ray Generator
• R-Axis IV++ Detector
• Varimax HR Osmic Mirrors
• Video Imaging System
• Haskris Chiller
• XStream 2000 N2 Generator System
• Dell Workstatio
I have ran into this problem before but cannot figure out how I worked
it out. I have setup ccp4-6.1.1 on a redhat enterprise linux box. I
have installed ccp4 under root and I can run things from my personal
account on the box. When I try and run something under an other
account then no
Hi Marek,
I had a similar problem before, which the active site was partially occupied
by a PEG molecule and my ligand couldn't get into the site. Sequential
soaking-out can solve my problem; basically you need to slowly remove the
PEGs from the crystal (in order to balance the osmotic shock): Ste
Hello,
A question about hardware. Assuming that size and noise and heat is more of
a problem than money, what would be the properties of the ideal lab X-ray
setup (source +optics + detector) for:
- protein single crystal diffraction work alone
- protein + small molecule crystals + powders + fiber
Dear All,
this is a request for opinion, relevant to HTPX operators such as
PSI centers, other large crystallography centers, or pharma HTP
pipelines. It is not really CCP4 related, so response perhaps directly
to me to avoid clutter.
We are making good progress on the robotic crystal harvesting,
A Postdoc and 2 PhD positions are available
in the Computational Structural Biology Group
at the Forschungszentrum Juelich, Germany
The group is developing computational methods for the structural
interpretation
of low-resolution and sparse experimental data with a focus on cryoEM.
Possible
Open positions in structural biology of cytoskeletal proteins at the
Helmholtz Centre for Infection Biology / DESY / University of Hamburg
Three positions funded by the Helmholtz Association are open in the
research group of Asst. Prof. Inari Kursula at the University of
Hamburg/DESY, Hambu
Dear Marek,
A lot may happen: Your active site might be blocked by PEG or crystal contacts,
or your sugar substrate gets converted into product and leaves the active site.
In your case I would try the following:
- soak or cocrystallize with a non-hydrolizable suger analog
- soak with as high a
Dear all,
I tried to soak substrate (sugar) into my enzyme-crystals. After solving the
structure I found PEG molecules instead of substrate in the active site. Does
this mean that the active site is blocked and the substrate isn´t able to get
into the enzyme-crsytals anymore? I used PEG 1000 i
Sorry a follow-up to this: the space-group number shown thus in the CIF
file:
_symmetry.Int_Tables_number 4005
_symmetry.space_group_name_H-M 'I 1 2 1'
should be shown as 5 NOT 4005! SG nos > 230 are 'CCP4-isms', and
International Tables knows nothing about them! I2 is merely an
altern
Ethan - that's odd it works for me (CCP4 6.1.0) unless of course it got
broken recently in 6.1.1:
First an extract from the mtz2various log file:
* Cell Dimensions : (obsolete - refer to dataset cell dimensions above)
70.3025 68.7834 93.7125 90. 94.1913 90.
* Resolution
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