Dear all, I tried to soak substrate (sugar) into my enzyme-crystals. After solving the structure I found PEG molecules instead of substrate in the active site. Does this mean that the active site is blocked and the substrate isn´t able to get into the enzyme-crsytals anymore? I used PEG 1000 in my crystallisation setups. Cocrystallisation showed no success so far.
Can anyone give some suggestion what I should do?
Thanks a lot,
Marek
