Hi,
Beautiful picture! It's too bad that the situation is nasty. You've got an
apparently young culture (the hyphae are small) however there's a large
quantity of free-floating individual cells, which strongly suggests that the
fungus may be dimorphic (or that the agitative motion of your culture
Hi,
Generally speaking, these days you don't need a special vector to introduce
a C-His (or an N-His, BAP, STREP, STREPII, or any short tag for that
matter), since the sequence of the tag + cleavage site is short enough to be
engineered directly into your PCR amplicon. There are numerous ways t
I had to marry into this knowledge, but ...
Begin forwarded message:
From: "Sara O'Rourke" <[EMAIL PROTECTED]>
Date: April 4, 2008 3:20:08 PM PDT
To: William Scott <[EMAIL PROTECTED]>
Subject: Re: [ccp4bb] Off-topic: fungus contamination of SF9 cells
[I] didn't send the picture but it sounds li
Dear Hari,
you also might want to specify, on the dm input LABOUT card,
the FCDM=FCDM and PHICDM=PHICDM coefficients, that output Fourier coefficients
avoiding the recombination with the initial phases, and will allow you to
inspect the truly-solvent-flattened, truly-averaged dm
Hi Filipp,
Start a new culture from your frozen cell bank using disposable
plasticwares. If it still happens and assuming the working environment is
clean and operators follow the SOP, you may have to get a new cell stock
from somewhere else.
We had a contamination once and traced back to its or
Dear all,
sorry for this off topic question. I am using baculovirus/insect cell
expression and I have had persisting problems with contamination that
seems to be some kind of fungus. I have a picture attached showing the
filamentous structure of it. This contamination has persisted for a
On Apr 4 2008, hari jayaram wrote:
Hi everyone, I have a phaser molecular replacement solution for my
membrane protein which crystallized in spacegroup P3. The diffraction
data is good to about 3.3 A. The model I used had 39% homology to the
given protein. A solvent content analysis suggests t
>In collagen a hydroxyl group is bonded to the CD atom of the prolyl ring to
>give an R configuration.
>[IUPAC: (2S,4R)-4-hydroxypyrrolidine-2-carboxylic acid]
>The monomer dictionary file HYP.cif incorrectly specifies the S
>configuration about the CD atom.
I've written CD where I should say CG
Dear All,
My colleague Erhard Hohenester has noticed that there is an error in the cif
definition file for hydroxyproline distributed with CCP4 version 6.02 and
used by Refmac, Coot (and Phenix).
In collagen a hydroxyl group is bonded to the CD atom of the prolyl ring to
give an R configuration.
As opposed to putting McDonald's "food" into their stomachs?
On Apr 4, 2008, at 9:33 AM, Ezra Peisach wrote:
I saw that toy - Personally, I do not like the idea of encouraging
children to aim a light (low powered led) directly at one's eye...
Ezra
P Hubbard wrote:
I took my 5 year old niec
Hi everyone,
I have a phaser molecular replacement solution for my membrane protein which
crystallized in spacegroup P3. The diffraction data is good to about 3.3 A.
The model I used had 39% homology to the given protein. A solvent content
analysis suggests that there probably are three dimers in t
I saw that toy - Personally, I do not like the idea of encouraging
children to aim a light (low powered led) directly at one's eye...
Ezra
P Hubbard wrote:
I took my 5 year old niece to a McDonald's (in England) for a happy
meal today - Spiderwick Chronicles promotion. The toy's light effect
Hi Vijay,
You may consider having a protease site in your 3' PCR primer, since you
probably have to remove the stop codon at the same time. It's probably
easier to do than finding a vector with C-terminal cleavable tag and C-His
will be in-frame without any extra sequence. Many pET vectors with
Hi,
I am looking for a vector with a cleavable C-terminal his tag for
prokaryotic protein expression. I found pET-52b but it has also got a
N-terminal cleavable strep-tag which I cannot avoid.
Thanks
Vijay
I took my 5 year old niece to a McDonald's (in England) for a happy meal today
- Spiderwick Chronicles promotion. The toy's light effect reminded me of a
visual aid I saw to help introduce X-ray diffraction and the crystal lattice to
students. My niece was more interested in using the visual ef
Posted on behalf of Jean-Luc Popot; please send enquiries/applications
to him directly ([EMAIL PROTECTED]) :
Position offer
Physico-Chimie Moléculaire des Membranes Biologiques,
CNRS/Université Paris 7 UMR 7099,
IBPC, 13 rue Pierre et Marie Curie, F-75005 Paris – France
Post-doctoral position
A two year post doctoral position is available at Leiden University, The
Netherlands (http://www.bfsc.leidenuniv.nl/) in computational methods
development. The position will be for the derivation and implementation
of new algorithms for exploiting all the available information in
macromolecular cr
Please try to use sfcheck binaries or source code from my home page:
http://www.ysbl.york.ac.uk/~alexei/sfcheck.html
Regards
Alexei
On 4 Apr 2008, at 09:58, Jovine Luca wrote:
On 4 Apr 2008, at 01:36, Michael Giffin wrote:
i get the same error on OS X 10.5.2 and 10.4.11:
sfcheck -f refm
The VIB Department of Molecular and Cellular Interactions at the Vrije
Universiteit Brussel is looking for a highly motivated X-ray
facility/computing manager. The successful candidate will provide expert
support to our in house structural biology community and will conduct
research in the area
On 4 Apr 2008, at 01:36, Michael Giffin wrote:
i get the same error on OS X 10.5.2 and 10.4.11:
sfcheck -f refmac2.mtz -m refmac2.pdb -out y -nomit 2 -map
the error:
Open failed: Unit: 11, File: sfcheck_scr.dat (logical:
sfcheck_scr.da
No. Better way is restraining internal degrees of freedom. Something
like elastic network model:
Tirion MM (1996) Phys Rev Letters 77 pp 1905-1908
But care should be taken with weights and which distances should be
restrained.
Fiixing position is not good way of using prior information (In
For SHELX there are two ways to fix selected atoms: either put AFIX 1
in front of the fixed atoms and AFIX 0 after them, or use the BLOC
instruction. e.g.
BLOC 1 N_1 > C_53 N_57 > C61 N_62 CA_62 C_62 O_62 N_63 > LAST
BLOC -1 N_1 > C_53 N_57 > C61 N_62 CA_62 C_62 O_62 N_63 > LAST
would fix resid
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