Hi everyone, I have a phaser molecular replacement solution for my membrane protein which crystallized in spacegroup P3. The diffraction data is good to about 3.3 A. The model I used had 39% homology to the given protein. A solvent content analysis suggests that there probably are three dimers in the ASU ( to give about 67% solvent) Phaser sucessfuly found two dimers in the ASU with good density and a third dimer with very weak almost "non-existent" density. I am now trying to do some NCS-averaging using DM to see If I can improve the desnity for dimer 3 and have a question about the different co-efficients I should be using for the averaging DM run.
Question1: The phaser output mtz file has a PHWT and a PHIC . For carrying out DM with flattening, averaging and histogram matching which phases do I use PHIC or PHWT along with observed Fo. Also for the weight do I use the FOM. Question2: The output mtz from DM run either way above , now has a PHIDM and a PHWT along with a FWT and FC. Which coefficients should I be using to get a map after DM for building. FWT and PHWT or Fo and PHIDM or FWT and PHIDM. Thank you for your help Hari
