Hi Rizwana,

There is no reason why we would expect the phylogenetic signal in the raw variables to be the same as the phylogenetic signal from the regression analysis (K or lambda), as in the regression, you are really looking at the phylogenetic signal of the _residuals_, which may be quite different. Also, the r-squareds from pgls in caper are not calculated in the same way as r-squareds from an ordinary least squares analysis. In fact, there is no "correct" way to calculate r-squared for any model other than the OLS model, as OLS r-squared is based on the residual variance. For other types of models, including GLS, "residual variance" is not a well-defined concept. Personally, I don't use r-squared for any model other than OLS models. There are far better ways to conduct model criticism.

Cheers,

Simon.

On 28/01/16 15:57, Rizwana Rumman wrote:
Dear R-sid-phylo list,

I am having some problems to interpret the results of a co-evolution analysis 
and I hope you can help me to get my head around things.

I have two variables that each show significant non-random phylogenetic signal (K 
and lambda) and appear to be significantly correlated in a non-phylogenetic 
regression; but when I am performing a pGLS in caper, the lambda is estimated to 
be zero and the two variables shows the exact same R-square and level of 
significance as estimated from the non-phylogenetic regression analysis. I also 
have two other sets of variables  that show lambdas >0 (very similar values 
from caper and ape) in the pGLS analysis, but for these variables, the R-square 
values are smaller from pGLS than those estimated from non-phylogenetic 
regression. I guess my question is if I can safely interpret that, in the first 
case, phylogenetic history does not significantly affect my trait correlations 
(indicated by lambda=0 and very similar simple linear regression and pGLS results) 
 and in the latter case (i.e. smaller R-squares after correcting for phylogeny), 
non-phylogenetic regressions slightly overes!
ti!
  mates the correlations of the traits.

Many thanks for the help!

Cheers,
Rizwana


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