So this is where I get confused on your intentions because you say that you want to do a voxel-wise analysis of an ROI. Do you mean you want to do a voxelwise analysis but only inside, eg, whole hippocampus?

On 1/12/2024 6:07 PM, Mirsol Choi wrote:

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Hello,

Sorry I should have clarified. Yes, I would like to perform voxelwise analysis of FA and MD values of hippocampal subfield between subjects. Is this possible with free surfer?

Thank you again for your time.

M

On Fri, Jan 12, 2024 at 4:05 PM Douglas N. Greve <dgr...@mgh.harvard.edu> wrote:

    oh, I thought you wanted to do an ROI analysis. You mean for a
    voxelwise analysis?

    On 1/12/2024 5:46 PM, Mirsol Choi wrote:

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    Hi there,


    Following the first registration step, should I also run another
    registration command to put all fa-in-subfields.mgz for each
    subject onto the same space for inter subject analysis of FA values?

    Thanks again,
    M

    On Fri, Jan 12, 2024 at 3:00 PM Douglas N. Greve
    <dgr...@mgh.harvard.edu> wrote:

        Map the diffusion data to the subfield space, eg,
        mri_vol2vol --mov fa.nii.gz --reg register.lta --targ
        subfields.mgz --o fa-in-subfields.mgz
        Then run mri_segstats
        mri_segstats  --ctab-default --i fa-in-subfields.mgz --seg
        subfields.mgz --sum fa-in-subfields.dat



        On 1/12/2024 4:53 PM, Mirsol Choi wrote:

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        Hi there,

        Is there a way to successfully obtain FA/MD values from the
        hippocampus subfields?

        Thank you,
        M

        On Fri, Jan 12, 2024 at 2:23 PM Douglas N. Greve
        <dgr...@mgh.harvard.edu> wrote:

            I'm not sure what you mean. The subfields are an
            ROI-based analysis. Are you saying you want to do a
            voxel-based analysis (without reference to ROIs)?

            On 1/9/2024 12:05 PM, Mirsol Choi wrote:

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            Hi there Freesurfer,

            I am currently using freesurfer to take DTI metrics
            (FA, MD) from hippocampal subfields.
            At this point of my exploration with freesurfer, I am
            able to correctly register the hippocampal subfields
            and diffusion data, and obtain my FA values from the
            subfields. However, this is at the individual subject
            level.

            I will eventually need to register all of my samples in
            the same space for me to do a group analysis. However I
            am unsure what the order of registration should be for
            this process to be feasible.

            Does this order seem feasible for accurate analysis?

            1) recon-all all of my subjects
            2) dt_recon all of my subjects (one of the outputs is a
            registration file, which has registered the DTI data to
            outputs of recon-all)
            3) run the hippocampus segmentation command on all
            subjects (segmentHA_T1.sh) to obtain hippocampal
            subfield segmentation
            4) register the segmentation file to the FA maps
            (output of dt_recon) using vol2subfield command
            5) After creating a subfield-FA map for each subject,
            spatial normalize the samples onto the same space using
            *mri_vol2vol*
            *
            *
            Thank you so much for taking the time reading my
            question. If there is a different method to spatially
            normalizing my datasets, or if there is a more optimal
            order of registration, I'd be happy to hear from you.

            Thank you,
            M

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