Thanks a lot Dr. Greve.

So as per your suggestion: I ran the command isxconcat-sess -sf sessidlist
-analysis workmem.sm05.rh -contrast encode-v-base -o my-group -all-contrasts
i.e.- without specifying -map argument. So this generates ces.nii.gz and
vesvar.nii.gz in output folder my-group.
To get the significance level from 2nd level analysis, I ran mri_glmfit --y
ces.nii.gz --wls cesvar.nii.gz --osgm --surface fsaverage lh --glmdir
my-glm.wls --nii.gz

This generates sig.nii.gz file. Could you please confirm once again, that's
how group level significant function connectivity is calculated from 1st
level FC maps?

Thanks a lot for all your help.



On Fri, May 6, 2016 at 3:23 PM, Douglas N Greve <gr...@nmr.mgh.harvard.edu>
wrote:

> You should not use the sig as that is the first level significance. If
> you pass that to the higher level, then the results will be hard to
> interpret. I think you'll want to use pcc or ces. Otherwise looks ok.
> doug
>
> On 05/06/2016 12:03 PM, Martin Juneja wrote:
> > Dear FS experts,
> >
> > I just finished calculating functional connectivity maps using
> > FreeSurfer. I just want to confirm if the following steps I used are
> > correct:
> >
> > (1). I defined configured seed regions parameters using fcseed-config
> > command, say R01.config and R02.config and created seeds using
> > fcseed-sess command to generate R01.dat and R02.dat files.
> > (2). Created nuisance vaiables: wm.config and vcsf.config files using
> > fcseed-config and -sess: this step is independent of seeds and is done
> > only once independent of seeds
> > (3). For mkanalysis-sess command for each seed using R0*.dat file, I
> > used '-notask' argument because I am performing resting-state analysis.
> > (4). For subject level analysis, I used selxavg3 for each seed
> > (5). For 2nd level analysis: isxconcat-sess -all-contrasts -map
> sig.nii.gz
> >
> > I can process all these steps without any error. If these steps are
> > correct, can some one please confirm again that for RSFC, I am
> > correctly using -notask in step (3) and -all-contrast in step (5).
> > Also, in the instructions, it's mentioned to use -map pcc if
> > interested in calculating partial correlation coefficient, but I am
> > interested in using sig to get group level significant maps, would
> > that be OK to use in this command as -map sig?
> >
> > Thanks for your help.
> >
> > Regards,
> > MJ
> >
> >
> > _______________________________________________
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> > Freesurfer@nmr.mgh.harvard.edu
> > https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
>
> --
> Douglas N. Greve, Ph.D.
> MGH-NMR Center
> gr...@nmr.mgh.harvard.edu
> Phone Number: 617-724-2358
> Fax: 617-726-7422
>
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