The fsaverage surface does not intersect very well with the MNI brain (the problem is that the average surface does not have as deep folds as an individual). You can see what is going on if you convert your label to fsaverage space with

tkmedit fsaverage orig.mgz -surfs -fmin 0.5 -overlay 1.nii -overlay-reg register.dat

This will show you the surfaces and the label. If the label does not intersect the surfaces, at all, then there's not much you can do. If there is some intersection, then you can run mri_vol2surf with --projfrac-max -.1 1.1 .1

doug


On 12/13/11 10:53 PM, vin . wrote:
#Registration
>tkregister2 --mov $d/T1.nii.gz --targ
           $d/mri/brain.mgz \
             --regheader --reg $d/vin_register.dat --noedit

I have cortical-clusters in 1mm MNI space so, I took 1mm MNI brain and registered this on the "fsaverage" default subject's /mri/brain.mgz"

Find attached register.dat

#checking registration
>
tkregister2 --targ $d/mri/brain.mgz \
      --mov T1.nii.gz --reg vin_register.dat

find attached register.tiff

Regards,
vin


On Wed, Dec 14, 2011 at 4:40 AM, Douglas Greve <gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>> wrote:

    Is your register.dat correct?


    On 12/13/11 10:33 PM, vin . wrote:
    Thanx Doug,

    atleast I see, now something. (see attached "seg.mgh -annot
    seg.annot.tiff)
    > tksurfer Sub_ID lh inflated -annot seg.annot -ov lh.seg.mgh
    -fthresh 0.01 -fmid 0.3 -fslope 1

    just to check, I also summed, all binary files. (attached
    sum_binarymap)
    >tksurfer Sub_ID  lh inflated -ov sum_binmap.nii -overlay-reg
    register.dat  -fthresh 0.01 -fmid 0.3 -fslope 1

    also find colortable.

    Greetings!

    On Wed, Dec 14, 2011 at 4:18 AM, Douglas Greve
    <gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>> wrote:

        Try tksurfer Sub_ID lh inflated -annot seg.annot
        doug


        On 12/13/11 9:39 PM, vin . wrote:
        no, in tksurfer, just inflated lh appears (no overlay).  so,
        I viewed lh.seg.mgh in freeview  and converted it to .nii
        and viewed in fslview.

        On Wed, Dec 14, 2011 at 3:37 AM, Bruce Fischl
        <fis...@nmr.mgh.harvard.edu
        <mailto:fis...@nmr.mgh.harvard.edu>> wrote:

            oh, so you mean you see a line on the surface?

            On Wed, 14 Dec 2011, vin . wrote:

                Thanx Bruce for quick reply.

                you are right, it's surface based file after
                mri_vol2surf.  yeah, tried with
                following command.

                >tksurfer Sub_ID lh inflated -annot lh.seg.annot -ov
                lh.seg.mgh -fthresh
                0.01 -fmid 0.3 -fslope 1


                On Wed, Dec 14, 2011 at 3:33 AM, Bruce Fischl
                <fis...@nmr.mgh.harvard.edu
                <mailto:fis...@nmr.mgh.harvard.edu>>
                wrote:
                     Hi Vin

                     it looks like your lh.seg.mgh is a
                surface-based .mgz not a
                     volume one. Did you try loading it into tksurfer?

                     cheers
                     Bruce

                     On Wed, 14 Dec 2011, vin . wrote:

                           Thank you Doug,

                >tkregister2 --mov $d/T1.nii.gz --targ
                           $d/mri/brain.mgz \
                             --regheader --reg $d/register.dat --noedit

                           after creating register.dat, I followed the
                           explained procedure,
                           Which resulted in "lh.seg.mgh". can't
                view this
                           file. ( appears a line )

                >mri_info lh.seg.mgh
                              Volume information for lh.seg.mgh
                                     type: MGH
                               dimensions: 163842 x 1 x 1 x 17
                              voxel sizes: 1.0000, 1.0000, 1.0000
                                     type: FLOAT (3)
                                      fov: 163842.000

                >mri_info 3b.nii
                           Volume information for 3b.nii
                                     type: nii
                               dimensions: 182 x 218 x 182
                              voxel sizes: 1.0000, 1.0000, 1.0000

                           #binary maps looks okay

                           may be I am doing mistake in registration or
                           mri_vol2surf ?
                >mri_vol2surf --reg register.dat --mov Nb.nii
                           --interp nearest --hemi lh --o
                           lh.Nb.mgh

                           Greetings!

                           On Tue, Dec 13, 2011 at 5:56 PM, Douglas
                N Greve
                <gr...@nmr.mgh.harvard.edu
                <mailto:gr...@nmr.mgh.harvard.edu>>
                           wrote:
                                It's a little involved but possible.
                                1. Binarize Nth cluster to have a
                binary value
                           of N
                                    mri_binarize --i N.nii --min 0.5
                --binval N
                           --o Nb.nii
                                2. Sample Nth cluster to the surface
                                    mri_vol2surf --reg register.dat
                --mov
                           Nb.nii --interp
                                nearest --hemi lh lh.Nb.mgh
                                After doing that will all clusters,
                combine
                           them together with
                                   mri_concat lh.1b.mgh lh.2b.mgh
                ... --vote
                           --o lh.seg.mgh
                                Now create a surface annotation
                                  mris_seg2annot --seg lh.seg.mgh
                --hemi lh --s
                           subject --o
                                lh.seg.annot --ctab yourctab
                                yourctab is a color table like
                                $FREESURFER_HOME/
                FreeSurferColorLUT.txt. You

                           list your regions
                                and give them the colors you want.

                                doug

                                vin . wrote:
                                      Hi Doug,
                                      sorry for confusing names.

                                      - 1.nii  is name of the
                cluster, which I
                           want to
                                      overlay. it's a coritical region,
                           resulted from
                                      fsl-probtrackx.
                                      I want to have few color codes
                in RGB
                                         R   G  B
                                      1. 255 0 255
                                      2. 116 0 116
                                      3. 0   0   255
                                      ...

                                      in this way, I would like to
                overlay 20
                           cortical
                                      regions in different colours
                on inflated
                           brain.

                                      -fthresh 0.3 here
                                      (1/(10^0.3) == 50.12 %
                                      in freesurfer email archieve,
                I found
                           this. hope
                                      it's correct.

                                      Thank you




                           On Mon, Dec 12, 2011 at 11:11 PM, Douglas
                N Greve
                <gr...@nmr.mgh.harvard.edu
                <mailto:gr...@nmr.mgh.harvard.edu>
                <mailto:gr...@nmr.mgh.harvard. edu
                <mailto:gr...@nmr.mgh.harvard.edu>>>
                           wrote:

                              Hi Vin, why do you say it's a 50%
                threshold? What
                           is the
                           1.nii
                              data? What colors do you want?
                              doug

                              vin . wrote:

                                  Dear FreeSurfer list,
                                  Greetings!

                                  a newbie to freesurfer.
                                  -- would like to overlay tracking
                group
                           results (Sum from
                           all
                                  subjects)-(from FSL ) in the
                inflated lh &
                           rh. --

                                  - it works with one region  (hope it's
                           correct), with
                                  following command, where, -fthresh
                ?? means
                           it shows 50 %
                                  threshold based on intensity /
                only voxels
                           which are
                           overlaid
                                  by atleast half of the subjects ??

                >tksurfer #Sub_ID lh inflated -annot
                           aparc.annot  -ov
                           1.nii
                                   -ovreg register.dat   -fthresh
                0.3 -fmid 0.3
                           -fslope 1

                                  - How I can overlay multiple
                regions, in
                           specific
                           colours,
                                  with 50% thr. ? ,
                                  Thank you :)
                                  Vin
                                  ----------------------------- -
                           ------------------------------
                                  ------------

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                              MGH-NMR Center
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                              The information in this e-mail is
                intended only
                           for the
                           person to
                              whom it is
                              addressed. If you believe this e-mail
                was sent to
                           you in
                           error and
                              the e-mail
                              contains patient information, please
                contact the
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                           MGH-NMR Center
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                <mailto:gr...@nmr.mgh.harvard.edu>
                           Phone Number: 617-724-2358
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