It's a little involved but possible.
1. Binarize Nth cluster to have a binary value of N
      mri_binarize --i N.nii --min 0.5 --binval N --o Nb.nii
2. Sample Nth cluster to the surface
      mri_vol2surf --reg register.dat --mov Nb.nii --interp nearest 
--hemi lh lh.Nb.mgh
After doing that will all clusters, combine them together with
     mri_concat lh.1b.mgh lh.2b.mgh ... --vote --o lh.seg.mgh
Now create a surface annotation
    mris_seg2annot --seg lh.seg.mgh --hemi lh --s subject --o 
lh.seg.annot --ctab yourctab
yourctab is a color table like $FREESURFER_HOME/FreeSurferColorLUT.txt. 
You list your regions and give them the colors you want.

doug

vin . wrote:
> Hi Doug,
> sorry for confusing names.
>
> - 1.nii  is name of the cluster, which I want to overlay. it's a 
> coritical region, resulted from fsl-probtrackx.
> I want to have few color codes in RGB
>     R   G  B
> 1. 255 0 255
> 2. 116 0 116
> 3. 0   0   255
> ...
>
> in this way, I would like to overlay 20 cortical regions in different 
> colours on inflated brain.
>
> -fthresh 0.3 
> here
> (1/(10^0.3) == 50.12 %
> in freesurfer email archieve, I found this. hope it's correct.
>
> Thank you
>
>
>
>
> On Mon, Dec 12, 2011 at 11:11 PM, Douglas N Greve 
> <gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>> wrote:
>
>     Hi Vin, why do you say it's a 50% threshold? What is the 1.nii
>     data? What colors do you want?
>     doug
>
>     vin . wrote:
>
>         Dear FreeSurfer list,
>         Greetings!
>
>         a newbie to freesurfer.
>         -- would like to overlay tracking group results (Sum from all
>         subjects)-(from FSL ) in the inflated lh & rh. --
>
>         - it works with one region  (hope it's correct), with
>         following command, where, -fthresh ?? means it shows 50 %
>         threshold based on intensity / only voxels which are overlaid
>         by atleast half of the subjects ??
>
>         >tksurfer #Sub_ID lh inflated -annot aparc.annot  -ov 1.nii
>          -ovreg register.dat   -fthresh 0.3 -fmid 0.3 -fslope 1
>
>         - How I can overlay multiple regions, in specific colours,
>         with 50% thr. ? ,
>         Thank you :)
>         Vin
>         ------------------------------ ------------------------------
>         ------------
>
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>
>     -- 
>     Douglas N. Greve, Ph.D.
>     MGH-NMR Center
>     gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>
>     Phone Number: 617-724-2358 <tel:617-724-2358> Fax: 617-726-7422
>     <tel:617-726-7422>
>
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>     <http://www.nmr.mgh.harvard.edu/facility/filedrop/index.html>
>
>
>
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-- 
Douglas N. Greve, Ph.D.
MGH-NMR Center
gr...@nmr.mgh.harvard.edu
Phone Number: 617-724-2358 
Fax: 617-726-7422

Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
FileDrop: www.nmr.mgh.harvard.edu/facility/filedrop/index.html

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