Hi Vin

it looks like your lh.seg.mgh is a surface-based .mgz not a volume one. Did you try loading it into tksurfer?

cheers
Bruce

On Wed, 14 Dec 2011, vin . wrote:

Thank you Doug,

>tkregister2 --mov $d/T1.nii.gz --targ $d/mri/brain.mgz \
  --regheader --reg $d/register.dat --noedit

after creating register.dat, I followed the explained procedure,
Which resulted in "lh.seg.mgh". can't view this file. ( appears a line )

>mri_info lh.seg.mgh
   Volume information for lh.seg.mgh
          type: MGH
    dimensions: 163842 x 1 x 1 x 17
   voxel sizes: 1.0000, 1.0000, 1.0000
          type: FLOAT (3)
           fov: 163842.000

>mri_info 3b.nii
Volume information for 3b.nii
          type: nii
    dimensions: 182 x 218 x 182
   voxel sizes: 1.0000, 1.0000, 1.0000

#binary maps looks okay

may be I am doing mistake in registration or mri_vol2surf ?
>mri_vol2surf --reg register.dat --mov Nb.nii --interp nearest --hemi lh --o
lh.Nb.mgh

Greetings!

On Tue, Dec 13, 2011 at 5:56 PM, Douglas N Greve <gr...@nmr.mgh.harvard.edu>
wrote:
      It's a little involved but possible.
      1. Binarize Nth cluster to have a binary value of N
          mri_binarize --i N.nii --min 0.5 --binval N --o Nb.nii
      2. Sample Nth cluster to the surface
          mri_vol2surf --reg register.dat --mov Nb.nii --interp
      nearest --hemi lh lh.Nb.mgh
      After doing that will all clusters, combine them together with
         mri_concat lh.1b.mgh lh.2b.mgh ... --vote --o lh.seg.mgh
      Now create a surface annotation
        mris_seg2annot --seg lh.seg.mgh --hemi lh --s subject --o
      lh.seg.annot --ctab yourctab
      yourctab is a color table like
      $FREESURFER_HOME/FreeSurferColorLUT.txt. You list your regions
      and give them the colors you want.

      doug

      vin . wrote:
            Hi Doug,
            sorry for confusing names.

            - 1.nii  is name of the cluster, which I want to
            overlay. it's a coritical region, resulted from
            fsl-probtrackx.
            I want to have few color codes in RGB
               R   G  B
            1. 255 0 255
            2. 116 0 116
            3. 0   0   255
            ...

            in this way, I would like to overlay 20 cortical
            regions in different colours on inflated brain.

            -fthresh 0.3 here
            (1/(10^0.3) == 50.12 %
            in freesurfer email archieve, I found this. hope
            it's correct.

            Thank you




On Mon, Dec 12, 2011 at 11:11 PM, Douglas N Greve
<gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>>
wrote:

   Hi Vin, why do you say it's a 50% threshold? What is the
1.nii
   data? What colors do you want?
   doug

   vin . wrote:

       Dear FreeSurfer list,
       Greetings!

       a newbie to freesurfer.
       -- would like to overlay tracking group results (Sum from
all
       subjects)-(from FSL ) in the inflated lh & rh. --

       - it works with one region  (hope it's correct), with
       following command, where, -fthresh ?? means it shows 50 %
       threshold based on intensity / only voxels which are
overlaid
       by atleast half of the subjects ??

       >tksurfer #Sub_ID lh inflated -annot aparc.annot  -ov
1.nii
        -ovreg register.dat   -fthresh 0.3 -fmid 0.3 -fslope 1

       - How I can overlay multiple regions, in specific
colours,
       with 50% thr. ? ,
       Thank you :)
       Vin
       ------------------------------
------------------------------
       ------------

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   --     Douglas N. Greve, Ph.D.
   MGH-NMR Center
   gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>
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MGH-NMR Center
gr...@nmr.mgh.harvard.edu
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