Dear Takanori, Thank you for your help. The issue is now resolved. I regret not recognizing the minor error earlier. I will be more careful about not sending screenshots of errors in the future
Best regards, Preeti On Wed, Mar 12, 2025 at 6:56 AM Takanori Nakane < tnakane.prot...@osaka-u.ac.jp> wrote: > Hi, > > Your file name contains space: "E1 11-2-25 URAC.tif". > This is not allowed. Please remove space from file names. > > Also please don't use screenshots to show textual error messages. > Copy and paste them as texts next time. > > Best regards, > > Takanori Nakane > > On 2025/03/11 22:18, Preeti wrote: > > Dear Takanori, > > > > Thank you for your help. I was doing exactly as you mentioned, but > > with RELION 4.0.1. I will try it with RELION 5.0 instead > > > > On Tue, Mar 11, 2025 at 5:19 PM Takanori Nakane <tnakane.protein@osaka- > > u.ac.jp <mailto:tnakane.prot...@osaka-u.ac.jp>> wrote: > > > > Hi, > > > > I cannot reproduce your problem. > > I did (with RELION 5.0): > > > > 1. Import: make sure to set "Are these multi-frame movies:" No. > > 2. Manual picking: select "Import/jobXXX/micrographs.star". > > > > Best regards, > > > > Takanori Nakane > > > > On 3/11/25 20:29, Preeti wrote: > > > Hello everyone, > > > > > > I am currently encountering an issue during the processing of > > negative > > > stain EM micrographs. I collected the raw images in |.tif| format > > during > > > negative stain EM imaging and intended to perform manual particle > > > picking using RELION. After successfully importing the > > micrographs, I > > > proceeded directly to manual particle picking, skipping both > motion > > > correction and CTF estimation steps. However, when attempting to > run > > > manual particle picking using the imported micrographs as input, > > RELION > > > returns an error stating that it 'cannot read the file or that > > the file > > > does not exist' — even though the files are clearly present and > > > functional, as Gaussian auto-picking (RELION) works without issue > > on the > > > same micrographs. I would appreciate any guidance or suggestions > to > > > resolve this issue, or recommendations for alternative software > > > platforms where I can perform manual particle picking on negative > > stain > > > micrographs > > > > > > Best regards, > > > Preeti > > > > > > > > > ------------------------------------------------------------------------ > > > > > > To unsubscribe from the CCP4BB list, click the following link: > > > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> > > > <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1>> > > > > > > > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
