Hi,
I cannot reproduce your problem.
I did (with RELION 5.0):
1. Import: make sure to set "Are these multi-frame movies:" No.
2. Manual picking: select "Import/jobXXX/micrographs.star".
Best regards,
Takanori Nakane
On 3/11/25 20:29, Preeti wrote:
Hello everyone,
I am currently encountering an issue during the processing of negative
stain EM micrographs. I collected the raw images in |.tif| format during
negative stain EM imaging and intended to perform manual particle
picking using RELION. After successfully importing the micrographs, I
proceeded directly to manual particle picking, skipping both motion
correction and CTF estimation steps. However, when attempting to run
manual particle picking using the imported micrographs as input, RELION
returns an error stating that it 'cannot read the file or that the file
does not exist' — even though the files are clearly present and
functional, as Gaussian auto-picking (RELION) works without issue on the
same micrographs. I would appreciate any guidance or suggestions to
resolve this issue, or recommendations for alternative software
platforms where I can perform manual particle picking on negative stain
micrographs
Best regards,
Preeti
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