Dear Takanori, Thank you for your help. I was doing exactly as you mentioned, but with RELION 4.0.1. I will try it with RELION 5.0 instead
On Tue, Mar 11, 2025 at 5:19 PM Takanori Nakane < tnakane.prot...@osaka-u.ac.jp> wrote: > Hi, > > I cannot reproduce your problem. > I did (with RELION 5.0): > > 1. Import: make sure to set "Are these multi-frame movies:" No. > 2. Manual picking: select "Import/jobXXX/micrographs.star". > > Best regards, > > Takanori Nakane > > On 3/11/25 20:29, Preeti wrote: > > Hello everyone, > > > > I am currently encountering an issue during the processing of negative > > stain EM micrographs. I collected the raw images in |.tif| format during > > negative stain EM imaging and intended to perform manual particle > > picking using RELION. After successfully importing the micrographs, I > > proceeded directly to manual particle picking, skipping both motion > > correction and CTF estimation steps. However, when attempting to run > > manual particle picking using the imported micrographs as input, RELION > > returns an error stating that it 'cannot read the file or that the file > > does not exist' — even though the files are clearly present and > > functional, as Gaussian auto-picking (RELION) works without issue on the > > same micrographs. I would appreciate any guidance or suggestions to > > resolve this issue, or recommendations for alternative software > > platforms where I can perform manual particle picking on negative stain > > micrographs > > > > Best regards, > > Preeti > > > > ------------------------------------------------------------------------ > > > > To unsubscribe from the CCP4BB list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> > > > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
