Hi Derek, you can do it in Phenix, a matter of one command or in GUI. Let me know off-list if you need guidance. Pavel
On Mon, Dec 16, 2024 at 6:39 AM Derek Logan < 0000ac2332bb2871-dmarc-requ...@jiscmail.ac.uk> wrote: > Hi everyone, > > I would like to do a quick rigid-body refinement of a model against a low > resolution (6 Å) cryo-EM map. The model consists of four tetramers and I > want to refine each tetramer as a rigid body, either with one > (anisotropic?) ADP per tetramer or at the most one per chain. I haven't yet > found a good way to do this. With phenix.refine the default is one ADP per > residue and I can't see how to change that behaviour (at least through the > Phenix GUI). I tried Servalcat but I can't see a way to restrict it to > rigid body only. Given more time, I hope to reach a fuller understanding of > each program, but I need to solve the immediate problem in the next couple > of days (what with Christmas coming up...) Any suggestions? > > I have also understood that the best practice is to refine against the > unsharpened map, correct? > > Thanks > Derek > _____________________________________________________ > *Derek Logan* > Professor | Biochemistry and Structural Biology > > Centre for Molecular Protein Science > Lund University, Box 124, 221 00 Lund, Sweden > www.cmps.lu.se > > > > > > > > > > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
