Hi everyone, I would like to do a quick rigid-body refinement of a model against a low resolution (6 Å) cryo-EM map. The model consists of four tetramers and I want to refine each tetramer as a rigid body, either with one (anisotropic?) ADP per tetramer or at the most one per chain. I haven't yet found a good way to do this. With phenix.refine the default is one ADP per residue and I can't see how to change that behaviour (at least through the Phenix GUI). I tried Servalcat but I can't see a way to restrict it to rigid body only. Given more time, I hope to reach a fuller understanding of each program, but I need to solve the immediate problem in the next couple of days (what with Christmas coming up...) Any suggestions?
I have also understood that the best practice is to refine against the unsharpened map, correct? Thanks Derek _____________________________________________________ Derek Logan Professor | Biochemistry and Structural Biology Centre for Molecular Protein Science Lund University, Box 124, 221 00 Lund, Sweden www.cmps.lu.se ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
