On Fri, 17 May 2024 08:27:02 -0700, James Holton <jmhol...@lbl.gov> wrote:
... > >With a bit of a caveat on how many photons/pixel you need for stable >background subtraction. XDS starts having issues around 1 photon/pixel >or less, and DIALS claims to be able to get to 0.01 photons/pixel, but I >have not personally pushed it that far. Not yet. > Hi James, I fully agree with your recommendations in general, but want to comment on this specific one, reporting the values that I wrote into the relevant XDSwiki article ( https://wiki.uni-konstanz.de/xds/index.php/Low_dose_data ; check out its history): - until 2015, the lowest average background count per pixel for XDS was 0.5 - in 2017 I changed that value to 0.05, after revision of the code in XDS (BUILT=20161101) To improve the estimate for very low background, one can use the RELRAD and RELBET keywords in XDS.INP . Best wishes, Kay ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/