Hi Marco, A few suggestions that I like to follow for MAD experiments:
Before everything, check you have at least about 1 SeMet per 100 residues Then before crystallisation check by MassSpec that SeMet is properly incorporated in your protein After crystallisation collect first on the peak with (very) high redundancy and as little/gentle dose as possible Collecting the other wavelengths should give you better starting phases/maps, which might be very helpful at your resolution of 2.8 (especially if it is a very anisotropic 2.8...)
Automated pipelines are so good nowadays, if you collect good data they should solve it without problems (as long as your crystal is not suffering from other pathologies like twinning or pseudosymmtries).
Good luck! D On 2024-05-14 01:17, Marco Bravo wrote:
CAUTION: This email originated from outside of the LMB: .-owner-ccp...@jiscmail.ac.uk-. Do not click links or open attachments unless you recognize the sender and know the content is safe. If you think this is a phishing email, please forward it to phish...@mrc-lmb.cam.ac.uk -- Hello all, I have a data collection trip next week and plan to collect data on selenomethionine derivative crystals at the al831 beamline. Are there any resources, tips, tutorials, literature etc. That you can recommend to help me prepare for these experiments. Also is there a way to plug in the experimental data into ccp4 cloud to do the automatic structure solution? Do I need native and derivative data to solve the structure? Last trip I collected a seemingly 2.8 angstrom resolution data on a crystal of the native protein but could not get a solution depsite extensive molecular replacement attempts. It seems that assigning a space group for the crystals has been troublesome as well. here is my last thread I posted about the issue for reference. https://www.jiscmail.ac.uk/cgi-bin/wa-jisc.exe?A2=ind2402&L=CCP4BB&O=D&X=CCE6DFA19FA3D40346&Y=mbrav005%40ucr.edu&P=112302 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
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