Hi,
One other option is to run MR in Phaser in one run with the tetramer made of the 4 monomers as model 1 and the single monomer as model 2. The gui offers this option and there is also an example in the documentaion.
Cheers,
Boaz
Boaz Shaanan, Ph.D.
Department of Life Sciences
Ben Gurion University of the Negev
Beer Sheva
Israel
Department of Life Sciences
Ben Gurion University of the Negev
Beer Sheva
Israel
On Feb 12, 2020 09:23, Rajnesh Kumari Yadav <rajn...@rcb.res.in> wrote:
Hello everyone,
I am working on a protein which have 5 domain in it, we have its 4 domain structure and 3.0 Angstrom data of 5 domain protein crystal. While doing the Molecular replacement we got solution with four domain with good electron density and space for last domain without any density. When we tried Molecular replacement with the missing domain (modelled) only we got solution shows density for this missing domain but not able see electron density for rest four domain even though room for 4 domain was visible in the solution. I need help or suggestion for this issue.
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I am working on a protein which have 5 domain in it, we have its 4 domain structure and 3.0 Angstrom data of 5 domain protein crystal. While doing the Molecular replacement we got solution with four domain with good electron density and space for last domain without any density. When we tried Molecular replacement with the missing domain (modelled) only we got solution shows density for this missing domain but not able see electron density for rest four domain even though room for 4 domain was visible in the solution. I need help or suggestion for this issue.
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