Thanks Clemens and Lumbini for your help. I had tried both the things but none of them worked for this problem.
On Wed, Feb 12, 2020 at 1:56 PM Clemens Vonrhein <vonrh...@globalphasing.com> wrote: > Hi, > > MOLREP [1] has a nice feature of searching for structures in electron > density - with the known parts of your model fixed. Basically follow > the recipe in [1] (search for "refmac.mtz" - but you can use any other > set of amplitudes/phases as well). In your case the 4-domain model > would be given to the -mx flag and the 5th domain search model to -m. > > This worked for me a few times in the past ... > > Cheers > > Clemens > > [1] http://www.ccp4.ac.uk/html/molrep.html > > On Wed, Feb 12, 2020 at 07:12:48AM +0000, Rajnesh Kumari Yadav wrote: > > Hello everyone, > > > > I am working on a protein which have 5 domain in it, we have its 4 > domain structure and 3.0 Angstrom data of 5 domain protein crystal. While > doing the Molecular replacement we got solution with four domain with good > electron density and space for last domain without any density. When we > tried Molecular replacement with the missing domain (modelled) only we got > solution shows density for this missing domain but not able see electron > density for rest four domain even though room for 4 domain was visible in > the solution. I need help or suggestion for this issue. > > > > ######################################################################## > > > > To unsubscribe from the CCP4BB list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > -- > > *-------------------------------------------------------------- > * Clemens Vonrhein, Ph.D. vonrhein AT GlobalPhasing DOT com > * Global Phasing Ltd., Sheraton House, Castle Park > * Cambridge CB3 0AX, UK www.globalphasing.com > *-------------------------------------------------------------- > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
