Hi, MOLREP [1] has a nice feature of searching for structures in electron density - with the known parts of your model fixed. Basically follow the recipe in [1] (search for "refmac.mtz" - but you can use any other set of amplitudes/phases as well). In your case the 4-domain model would be given to the -mx flag and the 5th domain search model to -m.
This worked for me a few times in the past ... Cheers Clemens [1] http://www.ccp4.ac.uk/html/molrep.html On Wed, Feb 12, 2020 at 07:12:48AM +0000, Rajnesh Kumari Yadav wrote: > Hello everyone, > > I am working on a protein which have 5 domain in it, we have its 4 domain > structure and 3.0 Angstrom data of 5 domain protein crystal. While doing the > Molecular replacement we got solution with four domain with good electron > density and space for last domain without any density. When we tried > Molecular replacement with the missing domain (modelled) only we got solution > shows density for this missing domain but not able see electron density for > rest four domain even though room for 4 domain was visible in the solution. I > need help or suggestion for this issue. > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 -- *-------------------------------------------------------------- * Clemens Vonrhein, Ph.D. vonrhein AT GlobalPhasing DOT com * Global Phasing Ltd., Sheraton House, Castle Park * Cambridge CB3 0AX, UK www.globalphasing.com *-------------------------------------------------------------- ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
