May be, I do co-relate your crystal pic with Manu Prakash at Stanford on his
work on Dancing Droplets, briefing the surface tension and evaporation ^ the
rule of two component fluids. # Since your precipitant contain PVP a shape
controlling agent
#https://med.stanford.edu/news/all-news/2015/03/researchers-solve-mystery-of-the-dancing-droplets.html
Best wishes
S.M.Jaimohan PhD
On Thursday, 28 March, 2019, 1:54:23 pm IST, Sergei Strelkov
<sergei.strel...@kuleuven.be> wrote:
#yiv3861306982 #yiv3861306982 --P{margin-top:0;margin-bottom:0;}#yiv3861306982
Artem (and Beatriz),
Me bad, could have thought about that! I think you are right.
There were initially bubbles in each drop (7 in one case, 4 in the other).
At some point the bubbles exploded (it was an instantaneous process, not just
shrinking).
Kind regards,
Sergei
Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of
Pharmaceutical Sciences, KU Leuven O&N2, Campus Gasthuisberg, Herestraat 49 bus
822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 32 Lab
pages: http://pharm.kuleuven.be/BiocrystallographyFrom: CCP4 bulletin board
<CCP4BB@JISCMAIL.AC.UK> on behalf of Artem Evdokimov <artem.evdoki...@gmail.com>
Sent: Thursday, March 28, 2019 1:07
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Neat!
Looks like multiple adjacent bubbles that were initially touching but
eventually shrunk down to the central cores - the connectors are protein
filaments (skin on the bubbles) left over from when bubbles had contact points.
Artem
On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville)
<bevan.marsh...@csiro.au> wrote:
Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition
is found in both Index and JCSG screens as well as Classics II.
Bevan Marshall
Staff Scientist | Collaborative Crystallisation Centre
Manufacturing
CSIRO
E bevan.marsh...@csiro.aut +61 3 9662 7492
343-351 Royal Parade, Parkville, VIC 3052
www.csiro.au|https://c3.csiro.au
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From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK]On Behalf Of LEGRAND
Pierre
Sent: Thursday, 28 March 2019 9:13 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
Dear Beatriz,
Nice drops :-))
Could it be that there is a reaction going on in these drops ?
The conditions are quite "exotic" with possibilities of coordination or
oxydoreduction (Co2+/Co3+) or polymerization...
Do you have reductants with the protein buffer ?
Is the protein an enzyme or a metalloprotein ?
Just some ideas.
Best wishes,
Pierre
De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Beatriz Gomes
Guimaraes [beatriz.guimar...@fiocruz.br]
Envoyé : mercredi 27 mars 2019 19:44
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] Interesting pattern on a crystallization drop
Dear all,
I would like to share with you a surprising pattern I found when examining some
crystallization plates (attached figures).
It is less obvious looking the photos, but apparently the "lines" are formed by
precipitated protein and there are some "bubbles" with small drops inside.I
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !
And it is not completely random because for the same condition the difference
between the two drops are : protein alone ("hexagon") and protein + ligand
("rhombus")
crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15
Have you seen anything similar before?
Thank you for your comments!
Beatriz
--------------------------
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
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