Hi Beatriz, Very interesting indeed. Couldn't it be the ghost printing of some mechanical part used to make the mould of the crystallization tray ? All the best, Philippe
Philippe BENAS, Ph.D. ARN UPR 9002 CNRS IBMC Strasbourg 15, rue René Descartes F-67084 STRASBOURG cedex +33.3.8841.7109 E-mails: p.be...@ibmc-cnrs.unistra.fr, philippe_be...@yahoo.fr URLs: http://www-ibmc.u-strasbg.fr/ , http://www-ibmc.u-strasbg.fr/spip-arn/ Le mercredi 27 mars 2019 à 19:54:42 UTC+1, Beatriz Gomes Guimaraes <beatriz.guimar...@fiocruz.br> a écrit : Dear all, I would like to share with you a surprising pattern I found when examining some crystallization plates (attached figures). It is less obvious looking the photos, but apparently the "lines" are formed by precipitated protein and there are some "bubbles" with small drops inside.I wish they were microcrystals but I do not think this is the case. I was suprised by the symmetry ! And it is not completely random because for the same condition the difference between the two drops are : protein alone ("hexagon") and protein + ligand ("rhombus") crystallization condition is: 0.01 M Cobalt(II) chloride hexahydrate 0.1 M Tris pH 8.5 20% w/v Polyvinylpyrrolidone K 15 Have you seen anything similar before? Thank you for your comments! Beatriz -------------------------- Beatriz Guimarães Laboratory of Structural Biology and Protein Engineering Instituto Carlos Chagas - ICC / FIOCRUZ Paraná Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C CIC 81350-010 Curitiba - PR, Brasil Tel.:+55(41)3316-3225/2104-3438 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
