Dear all,
some more details: drops were set up by a robot (Mosquito), 200 nL prot+ 200 nL reservoir on a 96-Well sitting drop iQ Plate (TTPlabtech). They are not really bubbles. I have the impression that the lines are all around creating these "empty rounds" (maybe "exploded bubbles"?) with the small drop inside. Beatriz -------------------------- Beatriz Guimarães Laboratory of Structural Biology and Protein Engineering Instituto Carlos Chagas - ICC / FIOCRUZ Paraná Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C CIC 81350-010 Curitiba - PR, Brasil Tel.:+55(41)3316-3225/2104-3438 ________________________________ De: Ailong Ke <ailong...@cornell.edu> Enviado: quarta-feira, 27 de março de 2019 17:06:01 Para: Beatriz Gomes Guimaraes Cc: CCP4BB@JISCMAIL.AC.UK Assunto: Re: [ccp4bb] Interesting pattern on a crystallization drop Hi Beatriz, Were the drops set up by hand or by a robot? If the latter, what kind of robot? are they hanging drops or sitting drops, and do the bubbles form on the glass surface of at the bottom of your drop? AK On Mar 27, 2019, at 2:44 PM, Beatriz Gomes Guimaraes <beatriz.guimar...@fiocruz.br<mailto:beatriz.guimar...@fiocruz.br>> wrote: Dear all, I would like to share with you a surprising pattern I found when examining some crystallization plates (attached figures). It is less obvious looking the photos, but apparently the "lines" are formed by precipitated protein and there are some "bubbles" with small drops inside. I wish they were microcrystals but I do not think this is the case. I was suprised by the symmetry ! And it is not completely random because for the same condition the difference between the two drops are : protein alone ("hexagon") and protein + ligand ("rhombus") crystallization condition is: 0.01 M Cobalt(II) chloride hexahydrate 0.1 M Tris pH 8.5 20% w/v Polyvinylpyrrolidone K 15 Have you seen anything similar before? Thank you for your comments! Beatriz -------------------------- Beatriz Guimarães Laboratory of Structural Biology and Protein Engineering Instituto Carlos Chagas - ICC / FIOCRUZ Paraná Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C CIC 81350-010 Curitiba - PR, Brasil Tel.:+55(41)3316-3225/2104-3438 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 <Drop2.jpg><Drop3.jpg> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
