If you mean you truncated the low resolution data to 5 angstrom, I wouldn't recommend that.
On Fri, Nov 3, 2017 at 12:24 PM, Eleanor Dodson <eleanor.dod...@york.ac.uk> wrote: > That map does not look like a 5A map? > I guess you mean something else.. > Eleanor > > On 3 November 2017 at 11:00, <herman.schreu...@sanofi.com> wrote: >> >> You are right. In this case, I would put some waters in it, refine and see >> if the density gets any clearer. However, since from this perspective the >> density is quite far away from the protein, it could be a very disordered >> PEG, which, even at high resolution, might be impossible to fit. >> >> >> >> Best, >> >> Herman >> >> >> >> Von: Abhishek Anan [mailto:rendezvous.a...@gmail.com] >> Gesendet: Freitag, 3. November 2017 11:10 >> An: Schreuder, Herman /DE >> Cc: CCP4BB@jiscmail.ac.uk >> Betreff: [EXTERNAL] Re: [ccp4bb] another unknown density problem >> >> >> >> Dear Prof Schreuder >> >> Here are another couple of perspectives from coot. The density is too far >> and isolated from the peptide chain to be an alternate conformation or >> conformational change. The density of the peptide chain does not look good >> because it was truncated at 5A for clarity. >> >> Best regards >> >> Abhishek >> >> >> >> On Fri, Nov 3, 2017 at 9:33 AM, <herman.schreu...@sanofi.com> wrote: >> >> Dear Abhishek, >> >> >> >> To me, it looks like an alternative conformation of the peptide chain or >> maybe even a conformational change with respect to the starting model. The >> peptide chain does not look too well defined, despite high resolution >> electron density. >> >> >> >> Best, >> >> Herman >> >> >> >> Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von >> Abhishek Anan >> Gesendet: Freitag, 3. November 2017 09:26 >> An: CCP4BB@JISCMAIL.AC.UK >> Betreff: [EXTERNAL] [ccp4bb] another unknown density problem >> >> >> >> Hi all, >> >> I have an "unknown" density in the map. I have tried to fit it to PEG but >> it doesn't fit very well. I was wondering if there are other PEG-related or >> other molecules I could try. >> >> The crystal grew in TRIS-HCl and PEG MME 2K. >> >> Thank you >> >> Abhishek >> >> > >
