Dear Pascal
have a look at : https://doi.org/10.1107/S0907444910002763 Acta Cryst. (2010). D66, 514-521 Macromolecular crystal data phased by negative-stained electron-microscopy reconstructions S. Trapani, G. Schoehn, J. Navaza and C. Abergel Best, --- Stefano Trapani Maître de Conférences http://www.cbs.cnrs.fr/index.php/fr/personnel?PERS=Stefano%20Trapani ------------------------------------- Centre de Biochimie Structurale (CBS) 29 rue de Navacelles 34090 MONTPELLIER Cedex, France Tel : +33 (0)4 67 41 77 29 Fax : +33 (0)4 67 41 79 13 ------------------------------------- Université de Montpellier CNRS UMR 5048 INSERM UMR 1054 ------------------------------------- Le 2016-10-25 03:49, Pascal Egea a écrit : > Dear All, > > I would like to know if it is possible to use a low resolution EM > reconstruction of a complex obtained in negative stain EM (not cryo EM) to > help molecular replacement in a 4.5A resolution X-ray diffraction data set of > the same complex > I am aware of the possibility of using low resolution cryoEM maps for MR as > described in the review from Jackson et al in Nature Protocols but I was > wondering if there is an intrinsically impossibility for negative stain > reconstructions. > > Any thoughts or advice will be greatly appreciated. > > Best, > -- > > Pascal F. Egea, PhD > Assistant Professor > UCLA, David Geffen School of Medicine > Department of Biological Chemistry > Boyer Hall room 356 > > 611 Charles E Young Drive East > Los Angeles CA 90095 > office (310)-983-3515 > lab (310)-983-3516 > email pegea at mednet.ucla.edu [1] > -- > This message has been scanned for viruses and > dangerous content by MAILSCANNER [2], and is > believed to be clean. Links: ------ [1] http://mednet.ucla.edu [2] http://www.mailscanner.info/ -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean.
