Dear Pascal, A while ago when I did my tests on MR at a very low resolution, I did it both with “shaped” envelopes and with the “flat” ones. It did work for both (with simulated data, which is obviously not the same as the experimental), but MR with the “shaped” models was more robust.
Some traces of this are in the article in Acta Cryst D51 (1995) 888-895. Best regards, Sacha Urzhumtsev De : CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] De la part de Pascal Egea Envoyé : mardi 25 octobre 2016 03:49 À : CCP4BB@JISCMAIL.AC.UK Objet : [ccp4bb] MR phasing using Negative Stain EM reconstruction Dear All, I would like to know if it is possible to use a low resolution EM reconstruction of a complex obtained in negative stain EM (not cryo EM) to help molecular replacement in a 4.5A resolution X-ray diffraction data set of the same complex I am aware of the possibility of using low resolution cryoEM maps for MR as described in the review from Jackson et al in Nature Protocols but I was wondering if there is an intrinsically impossibility for negative stain reconstructions. Any thoughts or advice will be greatly appreciated. Best, -- Pascal F. Egea, PhD Assistant Professor UCLA, David Geffen School of Medicine Department of Biological Chemistry Boyer Hall room 356 611 Charles E Young Drive East Los Angeles CA 90095 office (310)-983-3515 lab (310)-983-3516 email pegea at mednet.ucla.edu<http://mednet.ucla.edu>
