Dear All, I would like to know if it is possible to use a low resolution EM reconstruction of a complex obtained in negative stain EM (not cryo EM) to help molecular replacement in a 4.5A resolution X-ray diffraction data set of the same complex I am aware of the possibility of using low resolution cryoEM maps for MR as described in the review from Jackson et al in Nature Protocols but I was wondering if there is an intrinsically impossibility for negative stain reconstructions.
Any thoughts or advice will be greatly appreciated. Best, -- Pascal F. Egea, PhD Assistant Professor UCLA, David Geffen School of Medicine Department of Biological Chemistry Boyer Hall room 356 611 Charles E Young Drive East Los Angeles CA 90095 office (310)-983-3515 lab (310)-983-3516 email pegea at mednet.ucla.edu
