Hi CCP4eans, Do you guys have any preference in purifying a protein by SEC in FPLC system or using a solvent based HPLC system after the initial affinity column purification. Where would you prefer HPLC purification over standard FPLC? I have routinely seen HPLC based purification of organic molecules and small peptides but not so much of proteins. What in your experience are the Pros and Cons of each, in the field of protein purification? Any suggestions/insights welcome.
Thanks, Ivan
