Thanks everyone for your insights, I really appreciate it. Cheers, ivan On Mon, Apr 20, 2015 at 5:38 PM, Edward A. Berry <ber...@upstate.edu> wrote:
> On 04/20/2015 06:44 PM, xaravich ivan wrote: > >> Hi CCP4eans, >> >> solvent based HPLC system? >> > > Do you mean like acetonitrile:water:TFA on reverse-phase columns? I think > this is > routinely used for analytical runs or to obtain material for protein > chemistry, > but the concentration of acetonitrile required to elute protein from C8 > column > is high enough to unfold the protein, so it would have to be something you > can > refold to be useful for crystallography. > (I may be wrong, I may have misunderstood, and yes I realize water is a > solvent.) > > eab > > > On 04/20/2015 06:44 PM, xaravich ivan wrote: > >> Hi CCP4eans, >> >> Do you guys have any preference in purifying a protein by SEC in FPLC >> system or using a solvent based HPLC system after the initial affinity >> column purification. Where would you prefer HPLC purification over standard >> FPLC? >> I have routinely seen HPLC based purification of organic molecules and >> small peptides but not so much of proteins. >> What in your experience are the Pros and Cons of each, in the field of >> protein purification? >> Any suggestions/insights welcome. >> >> Thanks, >> Ivan >> >
