On 04/20/2015 06:44 PM, xaravich ivan wrote:
Hi CCP4eans,
solvent based HPLC system?
Do you mean like acetonitrile:water:TFA on reverse-phase columns? I think this
is
routinely used for analytical runs or to obtain material for protein chemistry,
but the concentration of acetonitrile required to elute protein from C8 column
is high enough to unfold the protein, so it would have to be something you can
refold to be useful for crystallography.
(I may be wrong, I may have misunderstood, and yes I realize water is a
solvent.)
eab
On 04/20/2015 06:44 PM, xaravich ivan wrote:
Hi CCP4eans,
Do you guys have any preference in purifying a protein by SEC in FPLC system or
using a solvent based HPLC system after the initial affinity column
purification. Where would you prefer HPLC purification over standard FPLC?
I have routinely seen HPLC based purification of organic molecules and small
peptides but not so much of proteins.
What in your experience are the Pros and Cons of each, in the field of protein
purification?
Any suggestions/insights welcome.
Thanks,
Ivan
