Or if the tip is hopelessly pitted you can get a new one (for a Branson 1/2
inch horn) at:
http://www.proequip.com/productlist.asp?pcid=31
(thanks to:
http://blogs.cornell.edu/collinslab/2010/04/12/time-for-a-new-tip/ )
Reza Khayat wrote:
A lot of "ageing" sonicators are not really ageing. Have you tried cleaning the
tip of the sonicator? If not, remove the tip from the sonicator and use a 800 Grit wet
sandpaper to make it as shiny as it was when you first bought it. I do this every three
times I use the sonicator. A lot of crap deposits on it with every use.
Best wishes,
Reza
Reza Khayat, PhD
Assistant Professor
The City College of New York
Department of Chemistry, MR-1135
160 Convent Avenue
New York, NY 10031
Tel. (212) 650-6070
---- Original message ----
Date: Tue, 4 Feb 2014 12:40:51 -0500
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> (on behalf of Roger Rowlett
<rrowl...@colgate.edu>)
Subject: Re: [ccp4bb] off-topic: bug busting
To: CCP4BB@JISCMAIL.AC.UK
BeadBeater. http://biospec.com/. Gentle,
aerosol-free way to break 15-350 mL of cell paste
(2-150 g wet packed cells).
Cheers,
_______________________________________
Roger S. Rowlett
Gordon & Dorothy Kline Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: rrowl...@colgate.edu
On 2/4/2014 11:49 AM, Phoebe A. Rice wrote:
Some time ago, there was a nice discussion of
cost-effective, wimpy protein-friendly ways to
break open E. coli. We're thinking about
replacing an aging sonicator. If people have a
favorite gizmo, could they repeat that advice?
thank you,
Phoebe Rice
++++++++++++++++++++++++++++++++++++++++++
Phoebe A. Rice
Dept. of Biochemistry & Molecular Biology
The University of Chicago
773 834 1723; pr...@uchicago.edu
http://bmb.bsd.uchicago.edu/Faculty_and_Research/
http://www.rsc.org/shop/books/2008/9780854042722.asp
