Govindjee and Fork's biography of Stacey French is available here:
http://www.nasonline.org/publications/biographical-memoirs/memoir-pdfs/french-c-stacy.pdf
or if your library subscribes to photosynthesis research,
http://link.springer.com/article/10.1007%2Fs11099-006-0041-6
Mark J van Raaij wrote:
LOL.
In any case, a French press is also not french, according to Wikipedia it was
invented by Charles Stacy French of the Carnegie Institution of Washington
(hence French press with capital F).
In any case, I agree, Emulsiflex, Constant Systems One-shot, Microfluidizer
etc. is the way to go if you have some money.
Mark J van Raaij
Lab 20B
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://www.cnb.csic.es/~mjvanraaij
On 4 Feb 2014, at 18:09, Pascal Egea wrote:
Hi Phoebe,
Another possibility is the Emulsiflex (from Avestin, in canada). Not a cheap
piece of equipment, but very sturdy and efficient to up to 200 mL of extract
(runs on house-air). Can deal with E coli (and even yeast if you have the
models with internal compressor). It comes in 3 sizes I think we have the
middle one (C-3) with a compressor. It is basically a french press but without
the inconvenient of being french (I am french myself). It is quite gentle, and
does not overheat samples as much as the sonicator. We make a lot of membrane
protein purifications and I have been working with this since my post-doc
Hope this helps.
Best regards,
Pascal Egea
On Tue, Feb 4, 2014 at 8:49 AM, Phoebe A. Rice <pr...@uchicago.edu> wrote:
Some time ago, there was a nice discussion of cost-effective, wimpy
protein-friendly ways to break open E. coli. We're thinking about replacing an
aging sonicator. If people have a favorite gizmo, could they repeat that
advice?
thank you,
Phoebe Rice
++++++++++++++++++++++++++++++++++++++++++
Phoebe A. Rice
Dept. of Biochemistry & Molecular Biology
The University of Chicago
773 834 1723; pr...@uchicago.edu
http://bmb.bsd.uchicago.edu/Faculty_and_Research/
http://www.rsc.org/shop/books/2008/9780854042722.asp
--
Pascal F. Egea, PhD
Assistant Professor
UCLA, David Geffen School of Medicine
Department of Biological Chemistry
Boyer Hall room 356
611 Charles E Young Drive East
Los Angeles CA 90095
office (310)-983-3515
lab (310)-983-3516
email pe...@mednet.ucla.edu
