3 to 1 ratio of bper and yper, 2-3 ml per gram of e. coli plus lysozyme and bensonaze... I have not used a sonicator in years :) about 1% of proteins dont like detergents, in which case there are other non mechanical options.
A. On Feb 4, 2014 10:49 AM, "Phoebe A. Rice" <pr...@uchicago.edu> wrote: > Some time ago, there was a nice discussion of cost-effective, wimpy > protein-friendly ways to break open E. coli. We're thinking about > replacing an aging sonicator. If people have a favorite gizmo, could > they repeat that advice? > thank you, > Phoebe Rice > > ++++++++++++++++++++++++++++++++++++++++++ > > Phoebe A. Rice > Dept. of Biochemistry & Molecular Biology > The University of Chicago > > 773 834 1723; pr...@uchicago.edu > http://bmb.bsd.uchicago.edu/Faculty_and_Research/ > > http://www.rsc.org/shop/books/2008/9780854042722.asp >
