A lot of "ageing" sonicators are not really ageing. Have you tried cleaning the tip of the sonicator? If not, remove the tip from the sonicator and use a 800 Grit wet sandpaper to make it as shiny as it was when you first bought it. I do this every three times I use the sonicator. A lot of crap deposits on it with every use.
Best wishes, Reza Reza Khayat, PhD Assistant Professor The City College of New York Department of Chemistry, MR-1135 160 Convent Avenue New York, NY 10031 Tel. (212) 650-6070 ---- Original message ---- >Date: Tue, 4 Feb 2014 12:40:51 -0500 >From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> (on behalf of Roger Rowlett ><rrowl...@colgate.edu>) >Subject: Re: [ccp4bb] off-topic: bug busting >To: CCP4BB@JISCMAIL.AC.UK > > BeadBeater. http://biospec.com/. Gentle, > aerosol-free way to break 15-350 mL of cell paste > (2-150 g wet packed cells). > > Cheers, > > _______________________________________ > Roger S. Rowlett > Gordon & Dorothy Kline Professor > Department of Chemistry > Colgate University > 13 Oak Drive > Hamilton, NY 13346 > > tel: (315)-228-7245 > ofc: (315)-228-7395 > fax: (315)-228-7935 > email: rrowl...@colgate.edu > > On 2/4/2014 11:49 AM, Phoebe A. Rice wrote: > > Some time ago, there was a nice discussion of > cost-effective, wimpy protein-friendly ways to > break open E. coli. We're thinking about > replacing an aging sonicator. If people have a > favorite gizmo, could they repeat that advice? > thank you, > Phoebe Rice > > ++++++++++++++++++++++++++++++++++++++++++ > > Phoebe A. Rice > Dept. of Biochemistry & Molecular Biology > The University of Chicago > > 773 834 1723; pr...@uchicago.edu > http://bmb.bsd.uchicago.edu/Faculty_and_Research/ > > http://www.rsc.org/shop/books/2008/9780854042722.asp
