Did anybody mention native gel electrophoresis to select suitable HA ions?
Worked for us really nicely in a situation were speed was essential.
Here's a reference: PMID 14646083
Klaus
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Dr. Klaus Fütterer
Room 717, Biosciences Tower
School of Biosciences P: +44-(0)-121-414 5895
University of Birmingham F: +44-(0)-121-414 5925
Edgbaston E: k.futte...@bham.ac.uk
Birmingham, B15 2TT, UK W: http://tinyurl.com/futterer-lab
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On 15 Jan 2014, at 20:49, Matthew Franklin wrote:
> Hi Rhys -
>
> Don't forget to try sulfur-SAD, especially the multi-crystal version
> published recently:
>
> http://journals.iucr.org/d/issues/2013/07/00/ba5189/index.html
>
> This seems well suited to your situation.
>
> - Matt
>
>
> On 1/15/14 12:18 PM, RHYS GRINTER wrote:
>> Hello message board,
>>
>> My group has some crystals of an interesting protein to take to the
>> synchrotron in a couple of weeks. We won't be able to prepare and
>> crystallise a SelMet derivative during that time period, but we have loads
>> of crystals sitting around. The diffraction isn't great, we see maybe 3.5 at
>> home but might be enough to get over the line.
>> It will be a very difficult MR target, so we were thinking of soaking so
>> crystals with heavy atomic compounds that we have lying around. I was
>> wondering if people had any suggestions of compounds that people have used
>> successfully for experimental phasing and maybe concentrations to use and
>> soaking time.
>>
>> Cheers,
>>
>> Rhys
>>
>
>
> --
> Matthew Franklin, Ph. D.
> Senior Scientist
> New York Structural Biology Center
> 89 Convent Avenue, New York, NY 10027
> (212) 939-0660 ext. 9374
